Journal of Plant Nutrition

Lee, Edward H.; Foy, Charles D.

doi: 10.1080/01904168609363544pmid: N/A

Abstract High‐performance liquid chromatography (HPLC) was used to determine aluminum (Al)‐induced changes in organic acid (OA) concentrations of Al‐tolerant ‘Dade’ and Al‐sensitive ‘Romano’ snapbean cultivars. Two week old ‘Dade’ and ‘Romano’ snapbean were grown in 1/5‐strength Steinberg nutrient solution for 10 days and then subjected to 0, 2, 4, 6, and 8 mg L‐1 Al treatments at pH 4.5 for an additional 3–15 days. Current studies confirmed earlier findings that the Dade cultivar was significantly more tolerant to Al than the Romano variety. Organic acid analyses were performed on extracts of root and leaf, and on stem exudates. The organic acids were separated on an ion exclusion column using a mobile phase of 0.01 N H3PO4. Individual OA were quantified with a variable wavelength detector operating at 210 nm. Aluminum stress tended to reduce the concentrations of citric, malonlc, malic, glycolic, fumaric, and acetic acids in the roots and increased the OA concentrations in stem exudates. In the presence or absence of Al stress, the Al tolerant Dade cultivar contained higher OA concentrations than did the Al‐sensitlve Romano. Aluminum stress reduced total OA levels in root extracts from Al‐sensltive Romano plants to a greater extent than in those of the Al‐tolerant Dade. Malic and citric acid concentrations were decreased more than those of the other organic acids examined. Results indicate that the Al‐tolerant Dade snapbean cultivar has a higher potential for Al‐chelation and detoxification than does the Al‐sensitive Romano. Hence, an Al‐chelation mechansism may be involved in differential Al tolerance within this species.

Edwards, J. H.; Pedersen, J. F.

doi: 10.1080/01904168609363545pmid: N/A

Abstract Five tall fescue (Festuca arundinacea Schreb.) clonal lines with diverse root and xylem diameters were grown in nutrient solutions with magnesium (Mg) concentrations of 42, 125 and 250 μM and potassium K concentrations of 133 and 333 μM. Leaf Mg concentrations increased with increasing Mg rates at both low and high K concentrations. The tall fescue line with the largest root and xylem diameters had low leaf Mg concentrations, indicating a possible increased Mg tetany potential when consumed by cattle. The response of the K/(Mg+Ca) ratio in the plant, an indicator of tetany potential, to varying solution Mg at low and high K was determined for each of the five lines. No Mg effects or interactions were significant. Line, K, and line x K effects were all significant for the K/(Mg+Ca) ratios. The line with the largest root and xylem diameters had the highest tetany potential (highest cation ratio). Higher solution K gave higher K/(Mg+Ca) ratios.

Grundon, N.J.; Asher, C.J.

doi: 10.1080/01904168609363546pmid: N/A

Abstract An apparatus was developed for the quantitative collection of volatile sulfur compounds released by intact plants and was used to study the effects of leaf temperature and stomatal aperture on the amount and pattern of release. An air stream sweeps volatiles released by the plants through a water‐cooled condenser system in which the air is dried prior to trapping the volatile sulfur compounds on activated carbon. Tests with 35S‐labelled 1‐butanethiol gave a mean recovery of 95.8 ± 4.3%. The yield of volatile sulfur compounds increased greatly when air flow rate increased from 1 to 2 1 min‐1 , but was independent of flow rate over the range 2 to 6 1 min‐1. About 93% of the trapped activity originated from plant shoots, about 1% from stem bases and roots and about 4% from culture solutions. Release of volatile sulfur compounds from intact plants followed a diurnal pattern, maximum rates occurring around midday and minimum rates overnight. Maximum rates of release ranged from 30 to 41 ng S g dry weight of shoots‐1 2 hr ‐1, while minimum rates ranged from 1.5 to 2.1 ng S g dry weight of shoots‐1 2hr‐1. Leaf temperature rather than stomatal aperture seemed to be the major factor controlling rate of release of volatile sulfur compounds. The rate of release was almost doubled by an increase of 7–9°C in leaf temperature.

Ramani, Saradha; Kannan, Seshadri; Patankar, A.V.

doi: 10.1080/01904168609363547pmid: N/A

Abstract The jute (Corchorus capsularis L.) cv. 3RC‐212 which is Fe‐efficient, was subjected to Fe‐deficiency stress, and the nutrient medium was examined for chemicals, when the plants became chlorotic and the pH was lowered to about 4. While phenolic acids could not be detected, DBP (dibutyl phthalate) was identified in the extract by means of TLC and HPLC. The effect of DBP and caffeic acid was studied in JRC‐212 and DBP was found to cause recovery of the plants from chlorosis in 5 days. The chemicals, PA (phthalic acid), a derivative of DBP (50 mg/1) were supplied to chlorotic plants of JRO‐632, an Fe‐inefficient jute cultivar, and both the chemicals were effective in chlorosis recovery. PA application caused more rapid greening than DBP. Jute is the second crop species in which DBP is identified in the root exudate. The detection of DBP was first recorded in sorghum CSH‐7.

Kannan, Seshadri

doi: 10.1080/01904168609363548pmid: N/A

Abstract The effects of DBP (Dibutyl phthalate) and PA (Phthalic acid) supplied to the nutrient medium of Fe‐deficiency stressed sorghum cultivars, CSH‐5, 2077‐A, and CS‐3541 were examined. It was found that both the chemicals (50 mg/1) caused recovery of the cultivars CSH‐5 and 2077‐A in 4 days of treatment. Furthermore, the growth of roots, especially the adventitious roots, was increased by the chemicals.

Ramani, Saradha; Kannan, Seshadri

doi: 10.1080/01904168609363549pmid: N/A

Abstract The absorption and transport of Na and Cl from 0.1 mM and 10 mM 22Na labelled NaCl or 36Cl labelled KCl were examined in 15 days old seedlings of 3 cultivars of rice differing in their tolerance to salinity. Furthermore, the effects of 10, 100 and 1000 ppm (N)2S on their uptake were studied. It was found that in general, the salt‐tolerant cultivars BR and PNL‐1 absorbed more Na and translocated a lesser proportion of it to the shoot, compared to the salt‐sensitive IR‐8, from 0.1 mM NaCl. The presence of (N)2S reduced the uptake of Na in all the cultivars. It was also found that the presence of 100 ppm K, KN or NNreduced Na absorption from 0.1 mM NaCl significantly in all the cultivars, and the translocation to shoot in BR‐ Chloride transport from 0.1 mM NaCl was reduced by (N)2S in all the cultivars. The 3 cultivars differed significantly in the rates of absorption and transport of Na and Cl. The results indicate that PNL‐1 which is a cross of IR‐8 X BR, has inherited the salt tolerance trait from BR. Lower rates of Na translocation to the shoot can be used as an index of salt tolerance in rice.

Coale, F. J.; Grove, J. H.

doi: 10.1080/01904168609363550pmid: N/A

Abstract The development of soybean (Glycine max (L.) Merr.) root systems was Investigated in two greenhouse pot experiments utilizing a modified cage technique. One soil at two levels of K availability was used to observe the effect of soil K fertility level on root weight and root length in 3 cm depth Increments to 24 cm. Experiments were terminated prior to restraint of root growth by the containers. Shoot mineral accumulation and dry matter partitioning between root and shoot components were Investigated. High K plants were shorter and had a greater root:shoot mass ratio than low K plants. A trend for greater root dry matter production in soil layers below 12 cm under high K conditions was observed. There were no differences in root length between the treatments at any depth. Tissue K content was greater in the high K treatments and this Increase was equivalents offset by decreased tissue Mg concentrations. The taller low K plants had a greater leaf area and a lower specific leaf weight, resulting in part from decreased starch content. Daily evapotranspirational water losses per pot tended to be greater under the low K availability regime. This Information led to the speculation that under low K conditions, the soybean plant may increase K accumulation by promoting transpirational water use, aiding soil K acquisition by mass flow and diffusion. Tissue carbohydrate analyses suggest greater translocation of photosynthate out of the leaf in the low K plants for use in root absorption metabolism, rather than for production of increased root dry matter and/or increased root length.

Boyer, Rodney F.; VanderPloeg, Jane R.

doi: 10.1080/01904168609363551pmid: N/A

Abstract Bean plants (Phaseolus vulgaris L.) were cultured in nutrient solutions containing three concentrations of iron. The leaves of plants grown with a limiting supply of iron contained reduced levels of protein, chlorophyll and the iron‐containing enzyme, lipoxygenase (EC 1.13.11.12). Measurements of lipoxygenase made on leaf crude extracts did not correlate with iron supply; however, upon purification of the enzyme by affinity chromatography, a direct correlation was observed. The lack of correlation was partly due to the presence of chlorophyll, an inhibitor of lipoxygenase, in the crude extract. Lipoxygenase levels changed with age of the plants, but rose to a maximum at days 14–16. A comparison of leaf lipoxygenases grown with different iron supply showed that they were identical with the same pH profile, chlorophyll inhibition, reaction products, and electrophoretic mobility. It is suggested that lipoxygenase may be used as a probe to evaluate iron availability and to investigate iron metabolism in higher plants.