SIMULTANEOUS LOCALIZATION OF MULTIPLE TISSUE ANTIGENS USING THE PEROXIDASE-LABELED ANTIBODY METHOD: A STUDY ON PITUITARY GLANDS OF THE RATNAKANE, PAUL K.
doi: 10.1177/16.9.557pmid: 5717715
The peroxidase-labeled antibody method was modified to localize multiple tissue antigens in a single histologic section. The first antigen was localized by the indirect method and the antisera were removed from the section by elution, leaving the colored reaction products identifying the antigenic sites. The second and subsequent antigens were localized similarly, using substrates that develop reaction products of different colors. For the demonstration of the method, anti-human growth hormone (GH), anti-human thyrotropic hormone (TSH) and anti-human chorionic gonadotropin, which cross-reacts with rat luteinizing hormone (LH), were used with sections of pituitary gland of the rat. Anti-GH reacted with small acidophilic cells. Anti-LH was localized in oval shaped cells, and anti-TSH was localized in angulated basophilic cells. Some cells were devoid of these three antigens and no cell contained more than one of them.
HYPERTROPHY OF LIVER CELL SMOOTH SURFACED RETICULUM FOLLOWING PROGESTERONE ADMINISTRATIONEMANS, JOHN B.; JONES, ALBERT L.
doi: 10.1177/16.9.561pmid: 5717716
Progesterone given daily for several days to male golden hamsters was shown to promote an increase in liver weight and a striking increase in hepatic smooth endoplasmic reticulum. This increase in smooth reticulum observed by electron microscopy was confirmed biochemically through microsomal phospholipid measurements. The alterations in liver structure brought about by administration of progesterone are comparable to those induced by phenobarbital. Hypertrophy of the smooth reticulum is seen in the form of a delicate system of interwoven tubules which freely anastomose and often are seen in confluence with the lamellar profiles of rough reticulum. Progesterone-induced hypertrophy of the hepatic smooth endoplasmic reticulum demonstrates that this organelle is responsive to an endogenous compound normally present in the circulation, and suggests that stimulation by steroids may be responsible in part for the maintenance of microsomal hydroxylases and smooth reticulum in the normal hepatic cell.
THE FINE STRUCTURAL LOCALIZATION OF ALKALINE AND ACID PHOSPHATASE ACTIVITY IN THE RAT SUBMANDIBULAR GLANDBOGART, BRUCE I.
doi: 10.1177/16.9.572pmid: 5717717
The lead capture method was employed to study the fine structural localization of nonspecific phosphatase activity in the rat submandibular gland. Alkaline phosphatase activity was observed in association with the plasma membrane and pinocytotic vesicles of the myoepithelial cell. A polarity in the distribution of alkaline phosphatase activity was described along the myoepithelial cell process. More reaction product was observed in association with the plasma membrane on the parenchymal surface than on the plamsa membrane on the stromal surface, where reaction product was confined mostly to the pinocytotic vesicles. Activity was evenly distributed over both surfaces of the portion of the myoepithelial cell characterized by the nucleus. Activity was also observed to be associated with pinocytotic vesicles of the endothelial cells and the plasma membrane of erythrocytes. No activity was observed in association with the ductal elements. Acid phosphatase activity was associated with membrane-bound structures in the acini and ducts. These structures took the form of lipofuscin granules in the acini and either multivesicular or dense bodies in the ducts.
A STUDY OF ARYL SULFATASE AND ACID PHOSPHATASE IN THE DEVELOPING NERVOUS SYSTEM OF THE RATCHOY, A. E.; CRAVIOTO, H.
doi: 10.1177/16.9.582pmid: 5717718
Aryl sulfatases A and B and acid phosphatase activities were studied in the nervous system of rats aged 5, 10, 15, 20 and greater than 45 days. The enzyme reaction products appeared as granules localized in neurons of the cerebral cortex, brain stem, choroid plexus and ependyma and in blood vessels. The distribution and intensity of enzyme reaction product varied with the age of the animals. Aryl sulfatase B activity appeared earlier and reached a maximum of intensity sooner than the other enzymes. Acid phosphatase and aryl sulfatase A activities appeared at the same time. However, acid phosphatase had a greater intensity of activity than aryl sulfatase A. The increased activity of aryl sulfatase B was parallel with the maturation of neurons. It is suggested that aryl sulfatase B may be an essential enzyme for myelination.