Journal of Histochemistry & Cytochemistry

MILLAR, M. J.; VINCENT, N. R.; MAWSON, C. A.

doi: 10.1177/9.2.111pmid: 14473913

A new method of preparing rats for 65Zn autoradiography has been described. By ensuring a low excretion rate of 65Zn, autoradiographs with good cellular detail were obtained. The method used for mounting stripping film prevented contact of water with the tissue except during floating-out of the sections.Autoradiographs of the lateral lobes of the dorsolateral prostate, epididymis, testis, ileum, pancreas and coat hair follicles are illustrated. 65Zn was concentrated at the base and lumen surfaces of the acinar cells in the lateral lobes of the dorsolateral prostate, in the tail pieces of testicular spermatozoa, in epididymal spermatozoa, in the Paneth cells of the ileum, in the pancreatic islets of Langerhans and in the shaft of the hair follicles. 65Zn distribution in liver, submaxillary glands, esophagus, pituitary, adrenals and kidney is also described.

NUKI, KLAUS; BONTING, SJOERD L.

doi: 10.1177/9.2.117pmid: 14480700

1. It has been possible to adapt the quantitative histochemical technique of microdissection and microchemical assay for the study of the quantitative distribution of alkaline phosphatase and lactic dehydrogenase activity in the first maxillary molar of the hamster, between birth and 4 days of age.2. The zones of the tooth buds dissected out and assayed were: stellate reticulum, stratum intermedium, ameloblasts, dentine plus enamel, odontoblasts, three areas of the dental papilla, and the cervical loop.3. The quantitative results for the alkaline phosphatase distribution were found to agree qualitatively with the results obtained by the Gomori and azo dye staining techniques, except that the staining techniques gave falsely negative reactions at low activities.4. Alkaline phosphatase activity was lowest in the zones furthest from dentine and enamel formation, while the two zones of cells most intimately concerned in the formation of dentine and enamel did not show the highest activity; in fact the ameloblast zone was one of the lowest in activity. The highest zones of activity, stratum intermedium and sub-odontoblastic papilla, were immediately next to the cells forming the matrices of dentine and enamel.5. These facts are considered to be supporting evidence for the theory of Neuman and Neuman regarding the "detoxifying" properties of alkaline phosphatase in a "barrier" surrounding the sites of calcification.6. The levels of lactic dehydrogenase activity were of the same order of magnitude in all the cellular zones. The zones furthest removed from enamel and dentine formation occupied the lower part of the range found.

DEB, CHANDICHARAN; MUKHERJI, MRITYUNJOY

doi: 10.1177/9.2.126pmid: N/A

1. A condition of reduced glomerular filtration and increased tubular reabsorption was produced experimentally in toads by the process of acute dehydration.2. In such a condition there was an interesting change, hitherto unrecorded, in acid phosphatase and plasmalogen content of the kidney. There was a fall in plasmalogen and a rise in acid phosphatase in kidney glomeruli during dehydration, and opposite results were observed in the tubules.3. It may be concluded that plasmalogen is related to the functional activity of the kidney; in the case of acid phosphatase, a non-functional accumulation has been observed.4. There was also a rise in succinic acid dehydrogenase and esterase in the functioning kidney tubules during dehydration.

GOLARZ, M. NELLY; BOURNE, GEOFFREY H.; RICHARDSON, H. DALE

doi: 10.1177/9.2.132pmid: 13899651

The proliferating connective tissue of muscular dystrophy can be shown by histochemical methods to contain enzymes which hydrolyse many high energy phosphates and phosphorylated co-enzymes, but which do not affect phosphorylated glycolytic intermediates. These results are discussed and the suggestion is made that the possibility of muscular dystrophy being primarily a connective tissue disease should be seriously considered.This work was carried out under a grant from the Muscular Dystrophy Associations of America Inc. and under a U. S. Public Health Service grant no. B2038. Some of the equipment with which the work was carried out was purchased with funds made available by the Aid to Muscular Dystrophy Research Chapter, Boston, Mass. of the National Foundation for Neuromuscular Diseases.

HAUMONT, S.

doi: 10.1177/9.2.141pmid: 13905408

High concentrations of zinc have been found by a histochemical method at the sites of calcification. Zinc has been detected in the developing osteons (Haversian systems) of compact bone exactly at the border line between calcified and uncalcified tissue (Fig. 1), in the cartilaginous partitions of hypertrophic cells (Fig. 3), and in the endochondral bone which has just been deposited in the metaphysis (Fig. 4).

BURSTONE, M. S.

doi: 10.1177/9.2.146pmid: 13875051

EISENSTEIN, R.; WERNER, M.; PAPAJIANNIS, S.; KONETZKI, W.; LAING, IRIS

doi: 10.1177/9.2.154pmid: 13889699

Chloranilic acid appears to be, for practical purposes, a specific histochemical reagent for the demonstration of calcium, forming colored crystals in the presence of calcium. Despite the disadvantages of only moderate sensitivity and relatively large crystal size, it proved to be quite useful for this histochemical identification of calcium.

GEORGE, J. C.; HEGDEKAR, B. M.

doi: 10.1177/9.2.157pmid: 13897754

Succinic dehydrogenase activity in the whole fat body of the desert locust and three species of grasshoppers has been demonstrated histochemically. The localization of the enzyme has been observed to be confined to the periphery of the cells. Fat solvents like ethyl acetate, ether, 3:1 ether-acetone mixture, or acetone alone, have been found to inhibit the enzyme, as was also the case with frozen sections or air dried material. Failure to demonstrate the activity of the enzyme in the latter case may be attributed to the possible release of an inhibitor, as has been already suggested by earlier authors in the case of homogenization.

AMANO, HARUKO; DAOUST, R.

doi: 10.1177/9.2.161pmid: 13860861

The distribution of ribonuclease activity in the liver of azo-dye fed animals was investigated histochemically using the substrate film method.The ribonuclease activity of liver parenchyma was lower than normal in cirrhotic and neoplastic rat livers while high activities were observed in the bile duct and connective tissue trabeculae. Intense reactions were also given by the necrotic regions.The decrease in ribonuclease activity of parenchymal cells was found to take place at early stages of 4-dimethylaminoazobenzene feeding, that is before hyperplastic cells are transformed into cancerous cells. No similar variation could be observed in the liver of animals fed the control basal diet nor in normal regenerating liver following partial hepatectomy.

RADDEN, B. G.

doi: 10.1177/9.2.165pmid: 14489723

1. In the mast cell, the staining of metachromatic granules is influenced by the pH of the solution—at a level of pH 3 metachromatic granules stain rapidly, and orthochromatic staining is retarded.2. The presence of metallic cations delays granule staining, the duration of the delay being proportional to the cationic concentration, related to the cationic valency, and to the position of the cation in its valency group.3. Granule staining is unaffected by the addition of metallic cations to the "pretreatment" solution, and accelerated by temperature increase.