journal article
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MICROMANIPULATION OF SALIVARY GLAND CHROMOSOMES
1942 Journal of Heredity
doi: 10.1093/oxfordjournals.jhered.a105087pmid: N/A
Abstract In larval salivary gland chromosomes of Chironomus plmosus, isolated by an osmic vapor technique, the following results were obtained by micro-manipulation: The chromosomes will withstand about 100 per cent elongation without permanent deformation, and about 300 per cent before breaking. Most of the elongation occurs in the inter-band regions. Breakage occurs straight across the chromosome at right angles to the long axis. There are indications of a volume increase during stretch. Under stress, diagonal and crisscross striations appear in the inter-band regions. In some instances these can be seen to be formed by the apposition of the walls of vesicles from an originally honeycomb-like structure. The striations are regarded as artifacts rather than genonemata. No evidence of any regular longitudinal differentiation was obtained in attempts to “shred” the chromosomes and to separate the synapsed homologs. After ether treatment, chromosomes are plastic, suggesting a possible role of lipoid in bonding the fabric. The elasticity, and certain other properties of salivary gland chromosomes suggest a tentative chemical comparison with those of fibrous proteins. A review of micromanipulation work on chromosomes is included. This content is only available as a PDF. Author notes *This work was done at the Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland, and at the Marine Biological Laboratory, Woods Hole, Mass., as part of a series of chromosome studies made in the laboratory of Dr. C. W. Metz with the support of the Rockefeller Foundation. The writer is much indebted to Dr. Metz for advice and encouragement The Carnegie Institution generously bore the c st of the plates, which were prepared with the aid of Mr. Chester Reather of that institution and Mr. Ray Maas and Mrs. H. D. Stalker of the University of Rochester. The assistance of Rochester colleagues and of Professors Chambers and Mast with the manuscript is acknowledged with pleasure. © Oxford University Press