doi: 10.1093/labmed/16.10.591pmid: N/A
Article PDF first page preview Close This content is only available as a PDF. © American Society of Clinical Pathologists
doi: 10.1093/labmed/16.10.594pmid: N/A
Article PDF first page preview Close This content is only available as a PDF. © American Society of Clinical Pathologists
doi: 10.1093/labmed/16.10.597pmid: N/A
Abstract Bilirubin has been classically fractionated into two chemically reactive forms—direct reacting and indirect reacting—by the diazo reaction. Although this has been useful in differentiating prehepatic from hepatic jaundice, the amounts of direct-reacting and total bilirubin measured are dependent on methodology. Recently, several chromatographic techniques have been introduced that can separate the mono- and diglucuronide conjugates of bilirubin, the unconjugated bilirubin, and an albumin-bound form of bilirubin. The clinical utility of these “new” fractions of bilirubin has yet to be fully established. This content is only available as a PDF. © American Society of Clinical Pathologists
Shulman, Ira, A.;Nelson, Janice, M.;Okamoto,, Mitzi;Malone, Sharon, A.
doi: 10.1093/labmed/16.10.602pmid: N/A
Abstract The importance of using commercial reagent red cells that always include a homozygous Jk(a + b − ) cell to screen patients′ sera for unexpected antibodies was evaluated. Reagent antibody screening cells consisting of a set of two single donor red cells were used throughout the study. Of 169,791 serum samples tested for unexpected antibodies, sera from 26 patients were found to contain anti-Jka, either alone (17 patients) or in combination with other unexpected antibodies (9 patients). During the first 31 months of the study only 44% of the patients′ sera was screened with sets of red cells that included a Jk(a + b − ) cell: a new anti-Jka was detected for every 9,976 sera tested and for every 5,890 patients transfused a delayed hemolytic transfusion reaction associated with anti-Jka occurred. During the following 20 months, 100% of the patients′ sera was screened with sets of red cells that included a Jk(a + b − ) cell: a new anti-Jka was detected for every 4,377 sera tested (P<0.05) and only one transfusion reaction was associated with anti-Jka after transfusing 12,080 patients. These data suggest that the omission of Jk(a + b − ) red cells from the antibody screening test could result in the failure to detect anti-Jka antibodies, which could potentially result in an increased incidence of hemolytic transfusion reactions associated with anti-Jka. It is recommended that at least one screening cell used for routine antibody detection methods be homozygous Jk(a + b − ). This content is only available as a PDF. © American Society of Clinical Pathologists
doi: 10.1093/labmed/16.10.605pmid: N/A
Article PDF first page preview Close This content is only available as a PDF. © American Society of Clinical Pathologists
doi: 10.1093/labmed/16.10.607pmid: N/A
Abstract Automated anatomic pathology data management systems have proliferated in recent years. These systems vary widely in their capabilities and costs, and must be evaluated on the basis of the data management requirements of individual laboratories. A distillation of basic system attributes was developed and arranged in a questionnaire format to help guide the process of selection and evaluation. A list of currently operational systems is also provided. This content is only available as a PDF. © American Society of Clinical Pathologists
Wright, Kevin, C.;Alan Wurth, Sister, M.
doi: 10.1093/labmed/16.10.611pmid: N/A
Abstract A comprehensive program for documenting pipette quality control using a radioassay procedure is discussed. The principles for testing both accuracy and precision are as follows: accuracy is determined by comparing the statistical means of counts from samples dispensed by the pipettes being tested to the mean of counts dispensed by a class A volumetric pipette. The difference is expressed as percent error. The precision of the device being tested is estimated from the variation of counts from samples dispensed and is expressed as the percent coefficient of variation. For institutions with existing radioassay facilities, this variation on the traditional radioassay procedure measures both quality control parameters without the expense of test kits or specialized equipment. The format illustrated provides a model of a comprehensive pipette quality control program. This content is only available as a PDF. © American Society of Clinical Pathologists
Flournoy, D., J.;Shirley, Ruth, Ann
doi: 10.1093/labmed/16.10.616pmid: N/A
Abstract Three different inoculum concentrations of common bacterial isolates were tested against currently used antimicrobial agents to determine the effect on zone sizes in the disk agar diffusion test. When zone sizes from inocula with 107 and 109 colony-forming units/mL were compared with zone sizes from inocula with 108 colony-forming units mL, 38.5% of the former displayed changes in diameter of at least 3 mm. Specific instances of the influence of the inoculum concentration on zone sizes for antimicrobial agents and organisms and the importance of these findings are discussed. This content is only available as a PDF. © American Society of Clinical Pathologists
doi: 10.1093/labmed/16.10.619pmid: N/A
Article PDF first page preview Close This content is only available as a PDF. © American Society of Clinical Pathologists
doi: 10.1093/labmed/16.10.627pmid: N/A
Article PDF first page preview Close This content is only available as a PDF. © American Society of Clinical Pathologists
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