British Journal of Dermatology

RALFKIÆR, ELISABETH; WANTZIN, G. LANGE; MASON, D. Y.; STEIN, H.; THOMSEN, K.

doi: 10.1111/j.1365-2133.1984.tb14146.xpmid: 6391533

SUMMARYSkin biopsies from nine patients with a histological and/or clinical diagnosis of cutaneous lymphocytoma, lymphoplasia or Jessner's lymphocytic infiltrate were examined by immunoenzymatic labelling with a panel of monoclonal antibodies against lymphocytes and accessory cells.Similar cellular constituents were demonstrated in the biopsies from three patients with lymphocytoma, two with lymphoplasia and two with atypical lymphocytes, but a protracted benign clinical course. The infiltrates from these patients consisted of T cells, Langerhans cells, related HLA‐DR positive dendritic dermal cells and clusters of polyclonal B cells. In four patients, the B cell clusters contained B cell accessory follicular dendritic cells, and thereby closely resembled the B cell follicles seen in lymphoid organs. The T cells were predominantly T helper/inducer cells and in all patients the T cells expressed HLA‐DR.One patient diagnosed as lymphocytoma cutis differed from the other patients by having no detectable B cells. One patient with Jessner's lymphocytic infiltrate differed from the other patients by having a marked relative predominance of T suppressor/cytotoxic cells.These data suggest that cutaneous lymphocytoma and lymphoplasia are basically similar disorders which may be considered to be exaggerated immune responses, whereas Jessner's lymphocytic infiltrate may be a separate entity. Immunological analysis may assist in establishing a definite diagnosis in cases of lymphocytoma or lymphoplasia with atypical cytological features.

SIEGENTHALER, G.; SAURAT, J.‐H.; MORIN, C.; HOTZ, R.

doi: 10.1111/j.1365-2133.1984.tb14147.xpmid: 6095888

SUMMARYThe distribution of cellular retinol‐ and retinoic acid‐binding proteins (CRBP and CRABP) in normal human epidermis and dermis was examined by gel filtration. We showed that CRBP is entirely bound to a lipid‐protein aggregate and its free form is obtained through delipidation. The type of homogenization procedure appeared to be important for the recovery of CRBP in human skin. The level of CRABP was about 3·1 pmol/mg protein in the epidermis whereas it was only sporadically detectable in the dermis. In contrast, CRBP was found in both tissues at a concentration of about 1 pmol/mg protein. The difference in CRABP concentrations between epidermis and dermis might have biological and therapeutic implications. Dissociation constants (Kd) of CRABP and CRBP were respectively 2·2 × 10−7 M and 2·51 × 10−7M. This method will facilitate the study of CRABP and CRBP in retinoid‐ responsive dermatoses and enable us to relate the therapeutic effects of retinoids to the levels of cellular retinoid‐binding proteins in the skin.

SCHMITT, D.; FAURE, M.; DAMBUYANT‐DEZUTTER, C.; THIVOLET, J.

doi: 10.1111/j.1365-2133.1984.tb14148.xpmid: 6210099

SUMMARYWe have used indirect immunogold electron microscopy to compare the respective density of cell membrane determinants revealed by OKT6 and OKT4 monoclonal antibodies on normal human Langerhans cells (LC): 12·9 ± 3·5 gold granules were noted per cell section on OKT4‐positive LC whereas 236·8 ± 23·5 granules were counted per cell section on OKT6‐reactive cells. These results confirm that human LC react with OKT4 antibody and they demonstrate a marked quantitative difference on LC surface between the antigenic determinants recognized by OKT6 and OKT4 antibodies.

DIFFEY, B.L.; OLIVER, R.J.; FARR, P.M.

doi: 10.1111/j.1365-2133.1984.tb14149.xpmid: 6508999

SUMMARYThe design and performance of an instrument for quantifying ultraviolet‐induced cutaneous erythema are discussed. If the instrument is used to record an‘erythema index’ at a site on the skin before and after irradiation, the difference between those two readings is essentially related to an increase in vasodilation and is largely independent of the melanin content of the epidermis.

FARR, P. M.; DIFFEY, B. L.

doi: 10.1111/j.1365-2133.1984.tb14150.xpmid: 6509000

SUMMARYA reflectance instrument was used to measure the variation in UVR‐induced erythema at different positions on the back. The pre‐irradiation erythema index decreased from top to bottom of the back but the increase in index remained constant for a fixed exposure dose. In contrast, the minimal erythema dose was higher at lower sites on the back. The measured erythemal response increased linearly with the logarithm of the radiation dose from approximately the minimal erythema dose up to at least fifteen times this value.

GAWKRODGER, D.J.; BEVERIDGE, G.W.

doi: 10.1111/j.1365-2133.1984.tb14151.xpmid: 6334534

SUMMARYA young woman with inactive discoid lupus erythematosus (LE) gave birth in three successive pregnancies to four male infants who showed cutaneous, and in one case cardiac, signs of neonatal LE. The mother had Ro and La antibodies although the anti‐nuclear factor (ANF) was not consistently detectable. Three of the infants received phototherapy for neonatal jaundice. Maternal discoid LE may give rise to neonatal LE, and successive siblings can be affected.

ROBERTSON, D.B.; KUBIAK, E.; GOMEZ, E.C.

doi: 10.1111/j.1365-2133.1984.tb14152.xpmid: 6239642

SUMMARYMultiple polypoid projections of granulation tissue developed in two patients receiving isotretinoin for acne. Histological study of the lesions revealed increased amounts of non‐sulphated acid mucopolysaccharides in the ground substance of the granulation tissue stroma. Complete resolution occurred following curettage with or without chemical cautery. The role of isotretinoin in the development of these lesions is discussed.

Potten, C.S.; Hume, W.J.; Parkinson, E.K.

doi: 10.1111/j.1365-2133.1984.tb14153.xpmid: 6509001

doi: 10.1111/j.1365-2133.1984.tb14154.xpmid: 6509002

Mitrani, Eduardo

doi: 10.1111/j.1365-2133.1984.tb14155.xpmid: 6509003