doi: 10.1080/00032718008055734pmid: N/A
Abstract This is a scanned image of the original Editorial Board page(s) for this issue.
Kato, Nobumasa; Ishii, Sumikazu; Naruse, Hiroshi; Irie, Minoru; Arakawa, Hidestoshi; Tsuji, Akio
doi: 10.1080/00032718008055735pmid: N/A
Abstract A method for enzyme imnunoassay of thyroid-stimulating hormone (TSH) in dried blood spotted onto filter paper has been developed. TSH was conjugated to horse-radish peroxidase according to Nakane's method. Separation of the bound and free fractions was obtained by a double antibody solid phase method using polyacetal beads which were coated with the purified IgG fraction from goat anti-rabbit IgG serum. p-Hydroxyphenyl propionic acid was used as substrate for the fluorophotometric assay of peroxidase activity. The assay sensitivity is 0.07, μU TSH/assay tube, which is equivalent to μU/ml when five 3 mm discs of dried blood spot are assayed. TSH values in dried blood samples obtained by this method correlate well with those of serum samples obtained by radioimmunoassay (r=0.89). The coefficients of variation were 6.8 to 13.4% (within assay) and 5 to 40% (between assay). The enzyme immunoassay of TSH presented here is applicable to the mass-screening for congenital hypothyroidism of neonate.
Mateescu, Mircea A.; Cornoiu, Irina; Schell, Horst D.
doi: 10.1080/00032718008055736pmid: N/A
Abstract The method described consists in the simultaneous determination of the action of the amylolytic preparations containing the associated α- and glucoamylase, upon native amylose (a substrate for both α- and glucoamylase) and upon cross-linked amylose (a substrate for α-amylase). In order to know the percentage of α- and glucoamylase respectively of the total amylolytic activity, the fallowing relations are proposed: %α = 2.31 Ax/A. 100 and % glucoamylase = 100 - %α, where A and Ax are the hydrolytic activity of the amylolytic preparation on nonmodified amylose and on cross-linked amylose respectively, The present method has an error of no more than 11%.
Sardesai, Vishwanath M.; Provido, Haydee S.
doi: 10.1080/00032718008055737pmid: N/A
Abstract A simple procedure for the determination of serine is described. The method involves the oxidation of serine to formaldehyde by periodate. The formaldehyde is then converted to 3,5-diacetyl - 1,4-dihydrolutidine by Hantzsch reaction in which acetyl acetone and ammonia are the reactants. The reaction product in low ranges (concentration of serine from 0.1 to 4 μg) is measured fluorometrically. In samples containing serine at concentrations higher than 4 μg colorimetric analysis is used. Recovery studies of serine added to washed mitochondrial preparations have been satisfactory. From the standpoint of sensitivity, simplicity and time required, this technique is an improvement over previously described procedures for serine determination.
Siskos, P. A.; Tzouwara, S. M.; Philianos, S. M.
doi: 10.1080/00032718008055738pmid: N/A
Abstract The spectrofluorimetric determination of kinetic parameters (Km, V and kobs) of conjugated bile acids with 3α-hydroxysteroid dehydrogenase from mutant Ps. Testosteroni is described. Under optimal pH conditions and pseudo- first order kinetics([β-NAD+)20 KmNAD+) the kinetic parameters of bile acids in different buffers are determined. The bile acids exhibit larger kinetic differences in glycine and Tris buffer compared to those measured in pvrophosphate buffer. From these data it is concluded that under certain conditions the differential kinetic determination of one bile acid in the presence of others is possible without prior separation.
Guilbault, G. G.; Chen, S. P.; Kuan, S. S.
doi: 10.1080/00032718008055739pmid: N/A
Abstract An extremely specific creatinine electrode was successfully developed for selective analysis of aqueous creatinine with a linear response over the range from 5 mg/l to 100 mg/l. The endogenous and exogenous species generally present in the serum do not interfere in the assay with the exception of ammonia. With the use of the electrode serum samples containing high creatinine can be assayed directly after pre-treatment. However, the electrode is not sensitive enough to detect the normal level serum creatinine due to the dilution of the serum after treatment.
Amer, Sawsan M.; Hassans, Sayed M.; El-tarras, Mohammed F.
doi: 10.1080/00032718008055740pmid: N/A
Abstract Two pH- induced difference- spectrophotometric procedures for the determination of oxyphenbutazone in pharmaceutical formulations are reported. Both procedures depend upon the sensitivity of the ultraviolet spectrum of oxyphenbutazone towards the pH of the solvent medium. In one procedure, the absorbance difference of oxyphenbutazone in an acid solvent (0.01 NHCl) and in an alkaline one (0.01 N NaOH) is measured at 254 nm; the mean percentage recovery amounts to 100.2±1.23 (p=0.05). The second procedure depends upon measurement of the absorbance difference of the drug in the acid solvent then in a pH 7-phosphate buffer at 262 nm; the mean percentage recovery amounts to 100.1±1.03 (p=0.05). The possible sources of interference in pharmaceutical formulations are studied and the two procedures are adapted to the analysis of some market preparations collected at random.
Tahir, Mohammad A.; Lateef, A. B.; Sarwar, M.
doi: 10.1080/00032718008055741pmid: N/A
Abstract A volumetric procedure for the determination of morphine in urine has been developed. The method is convenient, precise and accurate. N-bromosuccinimide has been used as a titrant using bordeaux red as indicator which changes from rose red to yellow at the end point. 1.51 to 9.06 mg morphine is determined from 100 ml of urine and the maximum relative standard deviation is 3% when 1.51 mg morphine is determined.
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