European Journal of Clinical Microbiology & Infectious Diseases

Castro-Lima, Victor A C; Santos, Ana Paula T; Musqueira, Priscila T; Maluf, Natalya Z; Ramos, Jéssica F; Mariano, Lívia; Rocha, Vanderson; Costa, Silvia F

doi: 10.1007/s10096-023-04613-wpmid: 37126131

The aim of this study was to evaluate the prevalence of latent Mycobacterium tuberculosis infection in hematopoietic stem cell transplantation candidates, using tuberculin skin test and QuantiFERON-TB Gold-Plus, in a high-burden tuberculosis country. Adult candidates for hematopoietic stem cell transplantation performed both tests before and those submitted to transplantation were followed up for 12 months. The prevalence of latent Mycobacterium tuberculosis infection was 17.1% and a moderate agreement between QuantiFERON-TB Gold-Plus and tuberculin skin test was observed in this population. Previous tuberculosis exposure was a risk factor for latent Mycobacterium tuberculosis infection. No cases of tuberculosis were diagnosed during follow-up period.

Piccinini, Daniele; Bernasconi, Enos; Carelli, Mattia; Luvini, Giorgia; Di Benedetto, Caroline; Lucchini, Gladys Martinetti; Barda, Beatrice; Bongiovanni, Marco

doi: 10.1007/s10096-023-04617-6pmid: 37133638

Parvimonas micra isolations are usually part of polymicrobial infections and the pathogenic role of this microrganism is still debated. We describe here a large series of hospitalized patients diagnosed with Parvimonas micra infections and discuss the clinical and therapeutic management and the outcome of these infections.

Corredoira, Juan; Miguez, Enrique; Mateo, Lara María; Fernández-Rodríguez, Ricardo; García-Rodríguez, José Francisco; Pérez-González, Alexandre; Sanjurjo, Ana; Pulian, María Victoria; Ayuso-García, Blanca; ,

doi:

Creti, Roberta; Imperi, Monica; Stanziale, Andrea; Giuliani, Giorgia; Fazii, Paolo; Savini, Vincenzo

doi: 10.1007/s10096-023-04620-xpmid: 37160573

A highly conserved fragment adjacent to the cfb gene encoding the CAMP factor is the target of PCR-based molecular diagnostic systems for the identification of S. agalactiae (group B streptococci (GBS)). Six PCR-negative, culture-positive GBS strains were whole genome sequenced to assess why they escaped molecular diagnostics. GBS strains did not constitute a clonal cluster and presented variably sized chromosomal deletions (from 7 to 33 kb) which always included the cfb gene, a finding never described before. GBS strains that escape molecular diagnostics are considered rare; however, they can cause false-negative results using molecular diagnostics alone, affecting medical decisions.

Di Bella, Stefano; Gatti, Milo; Principe, Luigi

doi: 10.1007/s10096-023-04612-xpmid: 37119346