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the theoretically modeled complex can help us to further understand the muticatalytic mechanism of anti-DNA antibodies. Until now, and to the best of our knowledge, this is the first report in the literature ...
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Research highlights &z.rtrif; A subset of anti-DNA antibodies contributes to CNS lupus. &z.rtrif; B cell receptor signal strength is governed by B cell intrinsic and extrinsic factors. &z.rtrif; Low ...
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vaccines induced accelerated and increased antibody responses compared to nontargeted vaccines. However, little is known about the molecular events on DCs after transfection with targeted DNA vaccines ...
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. In this work a DNA-based reconfigurable molecular aptamer beacon was developed. It is composed of two parts: a variable part and a constant part. The variable part comprises an aptamer strand and its ...
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(LCDR2)), and the five inter-protein molecular hydrogen bonds may profoundly impact the antibody–ssDNA interaction, a finding which may be useful for protein engineering of this antibody–ssDNA structure ...
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Highlights ► Genetic analysis of nine human monoclonal anti-HSP60 antibody Fab fragments isolated from peripheral blood lymphocytes of atherosclerosis patients. ► Somatic hypermutation and antigenic ...
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and on-blot digestion of the DNA-bound protein followed by LCâMS/MS, analysis provides better information about the molecular weight of protein [48, 49]. Non-radioactive methods for southern blotting make ...
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. Monoclonal antibodies that are prepared by using GroEL as a molecular adjuvant could be used in immunoassays, such as flow cytometry, western blotting, and immunoprecipitation, to detect both exogenous ...
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as capture antibody and an anti-tPSA mAb as detection antibody yields a sensitivity that is not sufficient for good reliability. This is probably due to the modest binding affinity of 5D3D11 for PSA ...