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for: design expression and characterization of a multivalent combination
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Design, expression, and characterization of a multivalent, combination HIV microbicide
Sexton, Amy; Harman, Sarah; Shattock, Robin J.; Ma, Julian K.-C.
Follow The FASEB Journal , Volume 23 (10): 3590
Fed of American Socs for Experimental Biology – Oct 1, 2009, S., Shattock, R. J., Ma, J. K.-C. Design, expression, and characterization of a multivalent, combination HIV microbicide. b12 cyanovirin-N fusion protein transgenic plants plant biotechnology ...
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Her2-specific Multivalent Adapters Confer Designed Tropism to Adenovirus for Gene Targeting
Dreier, Birgit; Mikheeva, Galina; Belousova, Natalya; Parizek, Petra; Boczek, Edgar; Jelesarov, Ilian; Forrer, Patrik; Plückthun, Andreas; Krasnykh, Victor
Follow Journal of Molecular Biology , Volume 405 (2)
Elsevier – Jan 14, 2011selected from rationally designed protein libraries and evolved to bind either the virus or the target receptor ( Fig. 1 b). A design was needed in which these modules could be combined in a multitude ...
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DARPins Recognizing the Tumor-Associated Antigen EpCAM Selected by Phage and Ribosome Display and Engineered for Multivalency
Stefan, Nikolas; Martin-Killias, Patricia; Wyss-Stoeckle, Sascha; Honegger, Annemarie; Zangemeister-Wittke, Uwe; Plückthun, Andreas
Follow Journal of Molecular Biology , Volume 413 (4)
Elsevier – Nov 4, 2011Designed Ankyrin Repeat Proteins (DARPins) represent a novel class of binding molecules. Their favorable biophysical properties such as high affinity, stability and expression yields make them ideal ...
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The Combination of Lectin Affinity Chromatography, Gel Electrophoresis and Mass Spectrometry in the Study of Plant Glycoproteome: Preliminary Insights
Markéta, Laštovičková; Dagmar, Smětalová; Janette, Bobalova
Follow Chromatographia , Volume 73 (1)
Springer Journals – Jun 1, 2011were one of the aims of this paper. This work combines affinity chromatography with 1D gel electrophoresis followed by mass spectrometry for the characterization of modified plant proteins. Lectin ...
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Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors
Shan, Yuebin; Luo, Ting; Peng, Chen; Sheng, Ruilong; Cao, Amin; Cao, Xueyan; Shen, Mingwu; Guo, Rui; Tomás, Helena; Shi, Xiangyang
Follow Biomaterials , Volume 33 (10)
Elsevier – Apr 1, 2012) and enhanced green fluorescent protein (EGFP), respectively for gene transfection studies. The Au DENPs/pDNA polyplexes with different N/P ratios and compositions of Au DENPs were characterized by gel ...
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Preparation and characterization of chitosan microparticles for oral sustained delivery of gliclazide: in vitro/in vivo evaluation
Barakat, Nahla S.; Almurshedi, Alanood S.
Follow Drug Development Research , Volume 72 (3)
Wiley – Oct 15, 2010drug–polymer combinations. The total dose of medication and the kinetics of release are the variables that can be manipulated to achieve the desired result. Microparticle‐based systems may increase ...
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Emerging Concepts on the Role of Innate Immunity in the Prevention and Control of HIV Infection
Ackerman, Margaret E.; Dugast, Anne-Sophie; Alter, Galit
Follow Annual Review of Medicine , Volume 63
Annual Reviews – Feb 18, 2012combined in this protocol. Another surprise was that protection was achieved in the absence of either a strong neutralizing antibody or CD8+ T cell response (2â4), prompting speculation ...
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Microarray‐based gene expression analysis as a process characterization tool to establish comparability of complex biological products: Scale‐up of a whole‐cell immunotherapy product
Wang, Min; Senger, Ryan S.; Paredes, Carlos; Banik, Gautam G.; Lin, Andy; Papoutsakis, Eleftherios T.
Follow Biotechnology and Bioengineering , Volume 104 (4)
Wiley – Sep 18, 2009analysis in combination with the herein introduced expression ratio with mean intensity (ERMI) analysis as useful tools for process characterization by global gene expression analysis. Biotechnol. Bioeng ...
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Generation of Bivalent and Bispecific Kringle Single Domains by Loop Grafting as Potent Agonists against Death Receptors 4 and 5
Lee, Chang-Han; Park, Kyung-Jin; Kim, Sang Jick; Kwon, Ohsuk; Jeong, Ki Jun; Kim, Ayeung; Kim, Yong-Sung
Follow Journal of Molecular Biology , Volume 411 (1)
Elsevier – Aug 5, 2011expression profiles and attenuated original target binding specificity and affinity due to undesirable interactions between the combined domains. 4 Conferring multivalency/multispecificity to a single domain ...
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The arginine mimicking β-amino acid β 3 hPhe(3-H 2 N-CH 2 ) as S1 ligand in cyclotheonamide-based β-tryptase inhibitors
Janke, Dennis; Sommerhoff, Christian P.; Schaschke, Norbert
Follow Bioorganic & Medicinal Chemistry , Volume 19 (23)
Elsevier – Dec 1, 2011cells are activated upon crosslinking of F c&z.epsiv; RI-bound IgE with multivalent allergens on the cells’ surface. The signaling cascade elicited eventually results in downstream events that trigger ...
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