Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/yap1-over-expression-in-saccharomyces-cerevisiae-enhances-glutathione-0cGFrM1sg9
References (13)
-
A. Delaunay, Anne‐Dominique Isnard, M. Tolédano (2000)
H2O2 sensing through oxidation of the Yap1 transcription factorThe EMBO Journal, 19
-
J. Rautio, K. Kataja, R. Satokari, M. Penttilä, H. Söderlund, M. Saloheimo (2006)
Rapid and multiplexed transcript analysis of microbial cultures using capillary electophoresis-detectable oligonucleotide probe pools.Journal of microbiological methods, 65 3
-
Yin Li, G. Wei, Jian Chen (2004)
Glutathione: a review on biotechnological productionApplied Microbiology and Biotechnology, 66
-
Jaekwon Lee, C. Godon, Gilles Lagniel, D. Spector, J. Garin, J. Labarre, M. Tolédano (1999)
Yap1 and Skn7 Control Two Specialized Oxidative Stress Response Regulons in Yeast*The Journal of Biological Chemistry, 274
-
A. Bachhawat, Dwaipayan Ganguli, J. Kaur, N. Kasturia, A. Thakur, H. Kaur, Akhilesh Kumar, A. Yadav (2009)
Glutathione Production in Yeast -
István Pócsi, Rolf Prade, Michel Penninckx (2004)
Glutathione, altruistic metabolite in fungi.Advances in microbial physiology, 49
-
D. Fracassetti, N. Lawrence, A. Tredoux, A. Tirelli, H. Nieuwoudt, W. Toit (2011)
Quantification of glutathione, catechin and caffeic acid in grape juice and wine by a novel ultra-performance liquid chromatography methodFood Chemistry, 128
-
K. Sugiyama, S. Izawa, Y. Inoue (2000)
The Yap1p-dependent Induction of Glutathione Synthesis in Heat Shock Response of Saccharomyces cerevisiae *The Journal of Biological Chemistry, 275
-
Y. Ueda, M. Yonemitsu, T. Tsubuku, M. Sakaguchi, R. Miyajima (1997)
Flavor characteristics of glutathione in raw and cooked foodstuffs.Bioscience, biotechnology, and biochemistry, 61 12
-
I. Nisamedtinov, K. Kevvai, Kerti Orumets, L. Arike, I. Sarand, M. Korhola, T. Paalme (2011)
Metabolic changes underlying the higher accumulation of glutathione in Saccharomyces cerevisiae mutantsApplied Microbiology and Biotechnology, 89
-
Guoyao Wu, Yun-Zhong Fang, Sheng Yang, J. Lupton, N. Turner (2004)
Glutathione metabolism and its implications for health.The Journal of nutrition, 134 3
-
Dominique Thomas, Y. Surdin-Kerjan (1997)
Metabolism of sulfur amino acids in Saccharomyces cerevisiaeMicrobiology and Molecular Biology Reviews, 61
-
Glen Wheeler, Eleanor Trotter, I. Dawes, C. Grant (2003)
Coupling of the Transcriptional Regulation of Glutathione Biosynthesis to the Availability of Glutathione and Methionine via the Met4 and Yap1 Transcription Factors*Journal of Biological Chemistry, 278
- Publisher
- Wiley
- Copyright
- Copyright © 2012 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1860-6768
- eISSN
- 1860-7314
- DOI
- 10.1002/biot.201100363
- pmid
- 22009669
- Publisher site
- See Article on Publisher Site
Abstract
Microbiological production of glutathione using genetically engineered yeast strains has a potential to satisfy the increasing industrial demand of this tripeptide. In the present work accumulation of glutathione in response to YAP1 over‐expression in Saccharomyces cerevisiae was studied. The over‐expression resulted in intracellular glutathione level over two times higher than in the parent strain. Transcript analyses revealed that, in addition to the genes encoding enzymes in the glutathione biosynthesis pathway (GSH1 and GSH2), the expression levels of the genes in the cysteine biosynthesis pathway (CYS3 and CYS4) were also significantly higher in the YAP1 over‐expressed strain. This suggests that YAP1 over‐expression affects glutathione accumulation at both its biosynthesis and substrate availability levels.
Journal
Biotechnology Journal – Wiley
Published: Apr 1, 2012
Keywords: ; ; ; ;