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A. Bøyum (1968)
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Instruction Manual: LeukoLOCK TM Total RNA Isolation System; Life Technologies
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Hemoglobin stimulates mononuclear leukocytes to release interleukin-8 and tumor necrosis factor alpha.Blood, 84 9
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M. Macey, D. McCarthy, T. Milne, J. Cavenagh, A. Newland (1999)
Comparative study of five commercial reagents for preparing normal and leukaemic lymphocytes for immunophenotypic analysis by flow cytometry.Cytometry, 38 4
The flow cytometric analysis of leukocytes in whole blood usually requires isolation of leukocytes from other components of whole blood. Density gradient centrifugation and red blood cell lysis are the most commonly used methods to separate leukocytes but come with significant limitations. We report the results of the evaluation of a microfabricated filtration device for blood preparation that separates erythrocytes from leukocytes based on their size and mechanical properties. The microfabricated filter evaluated here requires a rapid and simple procedure and results in high leukocytes recovery without introducing bias among the leukocyte subpopulations. The filter removes erythrocytes, platelets, plasma proteins, and unbound staining reagent. This gentle filtration process produces very clean stained leukocytes for cytometric analysis without any apparent damage to leukocytes. © 2011 International Society for Advancement of Cytometry
Cytometry Part A – Wiley
Published: Dec 1, 2011
Keywords: ; ;
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