Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Vibrational and electronic circular dichroism study of the interactions of cationic porphyrins with (dG‐dC) 10 and (dA‐dT) 10

Vibrational and electronic circular dichroism study of the interactions of cationic porphyrins... The interactions of two different porphyrins, without axial ligands—5,10,15,20‐tetrakis(1‐methylpyridinium‐4‐yl)porphyrin‐Cu(II) tetrachloride (Cu(II)TMPyP) and with bulky meso substituents—5,10,15,20‐tetrakis(N,N,N‐trimethylanilinium‐4‐yl)porphyrin tetrachloride (TMAP), with (dG‐dC)10 and (dA‐dT)10 were studied by combination of vibrational circular dichroism (VCD) and electronic circular dichroism (ECD) spectroscopy at different (oligonucleotide)/(porphyrin) ratios, where (oligonucleotide) and (porphyrin) are the concentrations of oligonucleotide per base‐pair and porphyrin, respectively. The combination of VCD and ECD spectroscopy enables us to identify the types of interactions, and to specify the sites of interactions: The intercalative binding mode of Cu(II)TMPyP with (dG‐dC)10, which has been well described, was characterized by a new VCD “marker” and it was shown that the interaction of Cu(II)TMPyP with (dA‐dT)10 via external binding to the phosphate backbone and major groove binding caused transition from the B to the non‐B conformer. TMAP interacted with the major groove of (dG‐dC)10, was semi‐intercalated into (dA‐dT)10, and caused significant variation in the structure of both oligonucleotides at the higher concentration of porphyrin. The spectroscopic techniques used in this study revealed that porphyrin binding with AT sequences caused substantial variation of the DNA structure. It was shown that VCD spectroscopy is an effective tool for the conformational studies of nucleic acid–porphyrin complexes in solution. © 2007 Wiley Periodicals, Inc. Biopolymers 85:349–358, 2007. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biopolymers Wiley

Vibrational and electronic circular dichroism study of the interactions of cationic porphyrins with (dG‐dC) 10 and (dA‐dT) 10

Biopolymers , Volume 85 (4) – Mar 1, 2007

Loading next page...
 
/lp/wiley/vibrational-and-electronic-circular-dichroism-study-of-the-HF35oEuk2Z

References (38)

Publisher
Wiley
Copyright
Copyright © 2007 Wiley Periodicals, Inc., A Wiley Company
ISSN
0006-3525
eISSN
1097-0282
DOI
10.1002/bip.20654
pmid
17167793
Publisher site
See Article on Publisher Site

Abstract

The interactions of two different porphyrins, without axial ligands—5,10,15,20‐tetrakis(1‐methylpyridinium‐4‐yl)porphyrin‐Cu(II) tetrachloride (Cu(II)TMPyP) and with bulky meso substituents—5,10,15,20‐tetrakis(N,N,N‐trimethylanilinium‐4‐yl)porphyrin tetrachloride (TMAP), with (dG‐dC)10 and (dA‐dT)10 were studied by combination of vibrational circular dichroism (VCD) and electronic circular dichroism (ECD) spectroscopy at different (oligonucleotide)/(porphyrin) ratios, where (oligonucleotide) and (porphyrin) are the concentrations of oligonucleotide per base‐pair and porphyrin, respectively. The combination of VCD and ECD spectroscopy enables us to identify the types of interactions, and to specify the sites of interactions: The intercalative binding mode of Cu(II)TMPyP with (dG‐dC)10, which has been well described, was characterized by a new VCD “marker” and it was shown that the interaction of Cu(II)TMPyP with (dA‐dT)10 via external binding to the phosphate backbone and major groove binding caused transition from the B to the non‐B conformer. TMAP interacted with the major groove of (dG‐dC)10, was semi‐intercalated into (dA‐dT)10, and caused significant variation in the structure of both oligonucleotides at the higher concentration of porphyrin. The spectroscopic techniques used in this study revealed that porphyrin binding with AT sequences caused substantial variation of the DNA structure. It was shown that VCD spectroscopy is an effective tool for the conformational studies of nucleic acid–porphyrin complexes in solution. © 2007 Wiley Periodicals, Inc. Biopolymers 85:349–358, 2007. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

Journal

BiopolymersWiley

Published: Mar 1, 2007

Keywords: electronic circular dichroism; vibrational circular dichroism; porphyrin; nucleotide; DNA ‐ porphyrin complexes, B ‐DNA, and Z ‐DNA

There are no references for this article.