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- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- Copyright
- Copyright © 2000 International Union of Biochemistry and Molecular Biology
- ISSN
- 1521-6543
- eISSN
- 1521-6551
- DOI
- 10.1080/15216540050022467
- pmid
- 10776597
- Publisher site
- See Article on Publisher Site
Abstract
A vanadium‐binding protein was isolated from the culture medium of the vanadium‐reducing bacterium Pseudomonas isachenkovii by utilizing vanadate as the terminal electron acceptor upon anaerobic respiration. The protein was associated with vanadium at a molar ratio of approximately 1:20. It was purified to homogeneity and separated into three components by treatment with 1 M HCl followed by gel filtration: a protein, a vanadium‐binding ligand, and inorganic vanadium. Electron paramagnetic resonance analysis showed that vanadium was associated with the protein in the 4+ oxidation state. The distribution of vanadium within the cell was studied by electron microscopy and x‐ray microanalysis of P. isachenkovii cells. The results suggest that vanadium, accumulated in special swells on the surface of the cell membranes, is reduced and excreted to the medium.
Journal
IUBMB Life – Wiley
Published: Feb 1, 2000
Keywords: Vanadium; Accumulation; Vanadium‐BINDING; Protein; Vanadate‐REDUCING; Bacteria