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Use of UV Absorbance To Monitor Furans in Dilute Acid Hydrolysates of Biomass

Use of UV Absorbance To Monitor Furans in Dilute Acid Hydrolysates of Biomass A simple method based on UV spectra was developed for the estimation of total furans (furfural and hydroxymethylfurfural) in hemicellulose hydrolysates. UV spectra of hemicellulose hydrolysate contained a single dominant peak at around 278 nm. Approximately two‐thirds of this peak can be attributed to furan absorbance (furfural and hydroxymethylfurfural). At 284 nm, both furfural and hydroxymethylfurfural have equal absorbance on a weight basis. A comparison of HPLC determinations for different samples of hydrolysate was used to develop a simple equation that allows the accurate prediction of total furans based on the difference in absorbance at 284 and 320 nm. This method may prove useful for quality control applications during the production of biomass syrups using a dilute acid hydrolysis process and during treatments for the amelioration of toxins. Although furans represent only a portion of the toxins present in hemicellulose hydrolysates, the abundance of furans appears to serve as a useful marker to predict relative toxicity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Progress Wiley

Use of UV Absorbance To Monitor Furans in Dilute Acid Hydrolysates of Biomass

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References (66)

Publisher
Wiley
Copyright
Copyright © 2000 American Institute of Chemical Engineers (AIChE)
ISSN
8756-7938
eISSN
1520-6033
DOI
10.1002/btpr.508
pmid
21312357
Publisher site
See Article on Publisher Site

Abstract

A simple method based on UV spectra was developed for the estimation of total furans (furfural and hydroxymethylfurfural) in hemicellulose hydrolysates. UV spectra of hemicellulose hydrolysate contained a single dominant peak at around 278 nm. Approximately two‐thirds of this peak can be attributed to furan absorbance (furfural and hydroxymethylfurfural). At 284 nm, both furfural and hydroxymethylfurfural have equal absorbance on a weight basis. A comparison of HPLC determinations for different samples of hydrolysate was used to develop a simple equation that allows the accurate prediction of total furans based on the difference in absorbance at 284 and 320 nm. This method may prove useful for quality control applications during the production of biomass syrups using a dilute acid hydrolysis process and during treatments for the amelioration of toxins. Although furans represent only a portion of the toxins present in hemicellulose hydrolysates, the abundance of furans appears to serve as a useful marker to predict relative toxicity.

Journal

Biotechnology ProgressWiley

Published: Jan 1, 2000

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