Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/ultraviolet-raman-spectroscopy-of-polyribouridylic-acid-excitation-CJS6xXBOpq
References (10)
-
G. Thomas, G. Medeiros, K. Hartman (1971)
The dependence of raman scattering on the conformation of ribosomal RNA.Biochemical and biophysical research communications, 44 3
-
L. Chinsky, P. Turpin, M. Duquesne, J. Brahms (1978)
Nucleic acid derivatives studied by preresonance and resonance Raman spectroscopy in the ultraviolet regionBiopolymers, 17
-
E. Small, W. Peticolas (1971)
Conformational dependence of the Raman scattering intensities from polynucleotidesBiopolymers, 10
-
M. Tsuboi, A. Hirakawa, Y. Nishimura, I. Harada (1974)
On the excited‐state geometry of the adenine residue as revealed by a preresonance Raman effectJournal of Raman Spectroscopy, 2
-
B. Tomlinson, W. Peticolas (1970)
Conformational dependence of Raman scattering intensities in polyadenylic acid.The Journal of chemical physics, 52 4
-
J. Tang, A. Albrecht (1970)
Developments in the Theories of Vibrational Raman Intensities -
E. Richards, C. Flessel, J. Fresco (1963)
Polynucleotides. VI. Molecular properties and conformation of polyribouridylic acidBiopolymers, 1
-
Exoch Small, W. Peticolas (1971)
Conformational dependence of the Raman scattering intensities from polynucleotides. III. Order‐disorder changes in helical structuresBiopolymers, 10
-
L. Chinsky, P. Turpin, M. Duquesne, J. Brahms (1977)
Structural investigation of poly d(BrU-A) by ultraviolet resonance raman spectroscopyBiochemical and Biophysical Research Communications, 75
-
N. Aylward, J. Koenig (1970)
Raman Spectra of Poly(adenylic acid)Macromolecules, 3
- Publisher
- Wiley
- Copyright
- Copyright © 1980 John Wiley & Sons, Inc.
- ISSN
- 0006-3525
- eISSN
- 1097-0282
- DOI
- 10.1002/bip.1980.360190807
- Publisher site
- See Article on Publisher Site
Abstract
Raman spectra of polyribouridylic acid excited in the uv region, from 363 to 290 nm, are reported. The conformational changes of the polymer from random coil to ordered structure with stacked bases at high and low temperature, respectively, are reflected by important changes in the Raman line intensities; this Raman hypochromism is itself a function of the excitation wavelength—its profile has been determined and shows negative values in the region of 290 nm (near resonance), i.e., hypochromism becomes hyperchromism. Thus the knowledge of the hypochromism excitation profile is important in following order–disorder transition of a polymer using resonance Raman spectroscopy. Theoretical attempts are proposed for explanation, involving not only the relative variations of the molar extinction coefficient on the order‐disorder transition of the polymer, but also the damping factors of the vibronic levels. The theoretical curve is found to fit adequately the experimental data over the excitation range, using only the frequency of the O‐O transition of uracil and a vibronic linewidth of 2200 cm−1.
Journal
Biopolymers – Wiley
Published: Aug 1, 1980