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R. Landers, U. Hübner, R. Schmelzeisen, R. Mülhaupt (2002)
Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering.Biomaterials, 23 23
D. Schmaljohann (2005)
Thermo-responsive polymers and hydrogels in tissue engineeringe-Polymers, 5
Laura Itle, M. Pishko (2005)
Cryopreservation of Cell‐Containing Poly(ethylene) Glycol Hydrogel MicroarraysBiotechnology Progress, 21
P. Vogt, J. Hauser, O. Rossbach, B. Bosse, W. Fleischer, H. Steinau, K. Reimer (2001)
POLYVINYL PYRROLIDONE‐IODINE LIPOSOME HYDROGEL IMPROVES EPITHELIALIZATION BY COMBINING MOISTURE AND ANTISEPIS. A NEW CONCEPT IN WOUND THERAPYWound Repair and Regeneration, 9
E. Wintermantel, J. Mayer, J. Blum, K. Eckert, P. Lüscher, M. Mathey (1996)
Tissue engineering scaffolds using superstructures.Biomaterials, 17 2
K. Lee, D. Mooney (2001)
Hydrogels for tissue engineering.Chemical reviews, 101 7
R. Pelham, Yu-li Wang (1997)
Cell locomotion and focal adhesions are regulated by substrate flexibility.Proceedings of the National Academy of Sciences of the United States of America, 94 25
Yu-li Wang, R. Pelham (1998)
Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells.Methods in enzymology, 298
A. Mikos, G. Sarakinos, Susan Leite, J. Vacanti, R. Langer (1993)
Laminated three-dimensional biodegradable foams for use in tissue engineering.Biomaterials, 14 5
A. El-Ghalbzouri, S. Gibbs, E. Lamme, C. Blitterswijk, M. Ponec (2002)
Effect of fibroblasts on epidermal regenerationBritish Journal of Dermatology, 147
Pierre Aymard, David Martin, Kevin Plucknett, Timothy Foster, Allan Clark, Ian Norton (2001)
Influence of thermal history on the structural and mechanical properties of agarose gels.Biopolymers, 59 3
C. Cochrane, M. Rippon, A. Rogers, R. Walmsley, D. Knottenbelt, P. Bowler (1999)
Application of an in vitro model to evaluate bioadhesion of fibroblasts and epithelial cells to two different dressings.Biomaterials, 20 13
Jeanie Drury, D. Mooney (2003)
Hydrogels for tissue engineering: scaffold design variables and applications.Biomaterials, 24 24
K. Lee, M. Peters, K. Anderson, D. Mooney (2000)
Controlled growth factor release from synthetic extracellular matricesNature, 408
H. Stark, M. Willhauck, N. Mirancea, K. Boehnke, Iris Nord, D. Breitkreutz, A. Pavesio, P. Boukamp, N. Fusenig (2004)
Authentic fibroblast matrix in dermal equivalents normalises epidermal histogenesis and dermoepidermal junction in organotypic co-culture.European journal of cell biology, 83 11-12
A. Balgude, Xiaojun Yu, A. Szymanski, R. Bellamkonda (2001)
Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures.Biomaterials, 22 10
(2005)
Wound Care Markets, 2nd Edn., Vol. 2, Burns
One of the stumbling blocks in the formation of a thermoresponsive cell‐hydrogel hybrid (TCH) is the efficient incorporation of cells in thermoresponsive hydrogels (TH) using traditional top‐down (i.e., cells penetrate in the pre‐set gels from top surface) approach. This approach is slow and tedious because the hydrogel needs to soak in the cells culture for a long time to allow cells to penetrate from the gel surface in to the bulk of the gel. In addition, cell incorporation into TH is difficult because elevated dissolution temperatures of gelators are detrimental to cell viability, whereas the highly viscous gel state that formed at ambient temperatures retards the penetration of cells. We propose a bottom‐up approach (i.e., cells mixed prior to gel setting) using a novel two‐step protocol. The first step is the rapid cooling of the agarose solution from its dissolution temperature (98°C) to 37°C and equilibrating for 3–4 min. The second step is the mixing of fibroblasts with the agarose solution and natural cooling to the room temperature to form the TCH. With this novel protocol, 90% of fibroblasts are found to be trapped in the cell‐gel hybrid.
Biotechnology Journal – Wiley
Published: Apr 1, 2006
Keywords: ; ; ;
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