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Transgenic mice carrying a tetracycline‐inducible, truncated transforming growth factor beta receptor (TβRII)

Transgenic mice carrying a tetracycline‐inducible, truncated transforming growth factor beta... The transforming growth factor‐betas (TGFβs) have multiple roles, making genetic analysis of their functions difficult. We therefore developed transgenic mouse lines to disrupt TGFβ signaling using a mechanism that is inducible, reversible, and cell‐type specific. The transgenic mouse lines carry an EGFP‐pBi‐DeltaTbetaRII construct (PTR). The ΔTβRII element codes for a dominant‐negative receptor that is known to disrupt TGFβ signaling. The ΔTβRII has a c‐myc tag. The transgene was silent in the PTR mice, with expression of both EGFP and ΔTβRII occurring when the PTR mice were crossed with mice that express the tetracycline transactivator (CMV‐tTA). The expression of EGFP was repressed by the addition of doxycycline to the drinking water of the PTRxCMV‐tTA mice. The PTR mice were then crossed with neuron‐specific‐tTA mice. Expression of the ΔTβRII transgene in these mice led to an upregulation of native TGFβ receptor expression, suggesting that neurons can modulate their responsiveness to TGFβs. genesis 42:1–5, 2005. © 2005 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genesis: the Journal of Genetics and Development Wiley

Transgenic mice carrying a tetracycline‐inducible, truncated transforming growth factor beta receptor (TβRII)

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References (29)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1526-954X
eISSN
1526-968X
DOI
10.1002/gene.20115
pmid
15828000
Publisher site
See Article on Publisher Site

Abstract

The transforming growth factor‐betas (TGFβs) have multiple roles, making genetic analysis of their functions difficult. We therefore developed transgenic mouse lines to disrupt TGFβ signaling using a mechanism that is inducible, reversible, and cell‐type specific. The transgenic mouse lines carry an EGFP‐pBi‐DeltaTbetaRII construct (PTR). The ΔTβRII element codes for a dominant‐negative receptor that is known to disrupt TGFβ signaling. The ΔTβRII has a c‐myc tag. The transgene was silent in the PTR mice, with expression of both EGFP and ΔTβRII occurring when the PTR mice were crossed with mice that express the tetracycline transactivator (CMV‐tTA). The expression of EGFP was repressed by the addition of doxycycline to the drinking water of the PTRxCMV‐tTA mice. The PTR mice were then crossed with neuron‐specific‐tTA mice. Expression of the ΔTβRII transgene in these mice led to an upregulation of native TGFβ receptor expression, suggesting that neurons can modulate their responsiveness to TGFβs. genesis 42:1–5, 2005. © 2005 Wiley‐Liss, Inc.

Journal

Genesis: the Journal of Genetics and Development – Wiley

Published: May 1, 2005

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