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Tissue‐engineered growth of bone by marrow cell transplantation using porous calcium metaphosphate matrices

Tissue‐engineered growth of bone by marrow cell transplantation using porous calcium... In this study we investigated not only osteoblastic cell proliferation and differentiation on the surface of calcium metaphosphate (CMP) matrices in vitro but also bone formation by ectopic implantation of these cell–matrix constructs in athymic mice in vivo. Interconnected porous CMP matrices with pores 200 μm in size were prepared to use as scaffolds for rat‐marrow stromal‐cell attachment. Cell–matrix constructs were cultured in vitro, and cell proliferation and ALPase activities were monitored for 56 days. In addition to their being cultured in vitro, cell–matrix constructs were implanted into subcutaneous sites of athymic mice. In vitro these porous CMP matrices supported the proliferation of osteoblastic cells as well as their differentiation, as indicated by high ALPase activity. In vivo the transplanted marrow cells gave rise to bone tissues in the pores of the CMP matrices. A small amount of woven bone formation was detected first at 4 weeks; osteogenesis progressed vigorously with time, and thick lamellar bones that had been remodeled were observed at 12 weeks. These findings demonstrate the potential for using a porous CMP matrix as a biodegradable scaffold ex vivo along with attached marrow‐derived mesenchymal cells for transplantation into a site for bone regeneration in vivo. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 216–223, 2001 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Materials Research Wiley

Tissue‐engineered growth of bone by marrow cell transplantation using porous calcium metaphosphate matrices

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Publisher
Wiley
Copyright
Copyright © 2000 John Wiley & Sons, Inc.
ISSN
1549-3296
eISSN
1552-4965
DOI
10.1002/1097-4636(200102)54:2<216::AID-JBM8>3.3.CO;2-3
Publisher site
See Article on Publisher Site

Abstract

In this study we investigated not only osteoblastic cell proliferation and differentiation on the surface of calcium metaphosphate (CMP) matrices in vitro but also bone formation by ectopic implantation of these cell–matrix constructs in athymic mice in vivo. Interconnected porous CMP matrices with pores 200 μm in size were prepared to use as scaffolds for rat‐marrow stromal‐cell attachment. Cell–matrix constructs were cultured in vitro, and cell proliferation and ALPase activities were monitored for 56 days. In addition to their being cultured in vitro, cell–matrix constructs were implanted into subcutaneous sites of athymic mice. In vitro these porous CMP matrices supported the proliferation of osteoblastic cells as well as their differentiation, as indicated by high ALPase activity. In vivo the transplanted marrow cells gave rise to bone tissues in the pores of the CMP matrices. A small amount of woven bone formation was detected first at 4 weeks; osteogenesis progressed vigorously with time, and thick lamellar bones that had been remodeled were observed at 12 weeks. These findings demonstrate the potential for using a porous CMP matrix as a biodegradable scaffold ex vivo along with attached marrow‐derived mesenchymal cells for transplantation into a site for bone regeneration in vivo. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 216–223, 2001

Journal

Journal of Biomedical Materials Research – Wiley

Published: Feb 1, 2001

Keywords: porous calcium metaphosphate (CMP) matrix; tissue engineering; marrow‐derived mesenchymal cells; transplantation; bone regeneration

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