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R. Klevecz, E. Stubblefield (1967)
RNA synthesis in relation to DNA replication in synchronized Chinese hamster cell cultures.The Journal of experimental zoology, 165 2
E. Robbins, P. Marcus (1964)
Mitotically Synchronized Mammalian Cells: A Simple Method for Obtaining Large PopulationsScience, 144
(1965)
Stepwise synthesis of DNA in diploid mammalian cells
K. Burton (1956)
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.The Biochemical journal, 62 2
E. Stubblefield (1964)
DNA SYNTHESIS AND CHROMOSOMAL MORPHOLOGY OF CHINESE HAMSTER CELLS CULTURED IN MEDIA CONTAINING N-DEACETYL-N-METHYLCOLCHICINE (COLCEMID)11Supported in part by Grant DRG-269 from Damon Runyon Memorial Fund for Cancer Research, and Grant E-286 from American Cancer Society, Inc.
E. Stubblefield, B. Brinkley (1967)
ARCHITECTURE AND FUNCTION OF THE MAMMALIAN CENTRIOLE
(1965)
Nucleic acid synthetic patterns in synchronized tumor cells
T. Hsu (1964)
MAMMALIAN CHROMOSOMES IN VITROThe Journal of Cell Biology, 23
E. Stubblefield (1966)
Mammalian chromosomes in vitro. XIX. Chromosomes of Don-C, a Chinese hamster fibroblast strain with a part of autosome 1b translocated to the Y chromosome.Journal of the National Cancer Institute, 37 6
M. Patterson, R. Greene (1965)
MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS.Analytical chemistry, 37
B. Brinkley, E. Stubblefield, T. Hsu (1967)
The effects of colcemid inhibition and reversal on the fine structure of the mitotic apparatus of Chinese hamster cells in vitro.Journal of ultrastructure research, 19 1
E. Stubblefield, R. Klevecz (1965)
Synchronization of Chinese hamster cells by reversal of colcemid inhibition.Experimental cell research, 40 3
V. Oyama, H. Eagle (1956)
Measurement of Cell Growth in Tissue Culture with a Phenol Reagent (Folin-Ciocalteau)Proceedings of the Society for Experimental Biology and Medicine, 91
(1966)
DNA , RNA , and protein synthesis in synchronized cultures of Chinese hamster fibroblasts
P. Lindahl, L. Sörenby (1966)
A new method for the continuous selection of cells in mitosis.Experimental cell research, 43 2
T. Terasima, L. Tolmach (1963)
Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells.Experimental cell research, 30
G. Mueller, K. Kajiwara, E. Stubblefield, R. Rueckert (1962)
Molecular events in the reproduction of animal cells. I. The effect of puromycin on the duplication of DNA.Cancer research, 22
E. Vischer, E. Chargaff (1948)
The separation and quantitative estimation of purines and pyrimidines in minute amounts.The Journal of biological chemistry, 176 2
R. Rueckert, G. Mueller (1960)
Studies on unbalanced growth in tissue culture. I. Induction and consequences of thymidine deficiency.Cancer research, 20
E. Stubblefield, B. Brinkley (1966)
CILIA FORMATION IN CHINESE HAMSTER FIBROBLASTS IN VITRO AS A RESPONSE TO COLCEMID TREATMENTThe Journal of Cell Biology, 30
10.1002/jcp.1040690311.abs Chinese hamster fibroblasts in monolayer cultures were synchronized by accumulating mitotic cells in the presence of Colcemid, removing the mitotic cells with a brief trypsin treatment, and growing them in medium lacking Colcemid. Such cultures grew normally and exhibited no significant deviations from control cultures in their mitotic interval, generation time, DNA synthesis kinetics, or proliferative capacity. The macromolecular composition of 106 mitotic cells was chemically determined to be: DNA, 15 μg; RNA, 28 μg; and protein, 190 μg. In stock cultures, the corresponding values were about 60% to 70% of those for mitotic cells. The kinetics of DNA, RNA, and protein synthesis were measured throughout a 12‐hour cell cycle by incorporation of tritiated precursors. DNA synthesis began two hours after, and continued until ten hours after Colcemid recovery, with 40 minute interruptions at five and eight hours. RNA synthesis commenced at one hour and continued linearly until the fifth hour, at which time the rate abruptly doubled. Protein synthesis began immediately after cell division (0.5 hour) and continued linearly until the sixth hour, at which time its rate also doubled. The simplest interpretation of the data suggests that most of the DNA involved in transcription was replicated in the first third of the DNA synthesis period. Thereafter, the rates of RNA and protein synthesis increased because of the doubling of the active template population in each cell.
Journal of Cellular Physiology – Wiley
Published: Jun 1, 1967
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