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- Publisher
- Wiley
- Copyright
- Copyright © 2007 Wiley Periodicals, Inc., A Wiley Company
- ISSN
- 0006-3525
- eISSN
- 1097-0282
- DOI
- 10.1002/bip.20655
- pmid
- 17167795
- Publisher site
- See Article on Publisher Site
Abstract
Nanocrystalline semi‐conductor materials, called quantum dots (QDs), exhibit unique optical and spectroscopic properties, which include, broad absorption, narrow and tunable emission, resistance to photobleaching, strong luminescence, and long luminescent lifetimes. These remarkable properties of QDs have resulted in their use as an alternative to both small‐molecule and protein fluorophores in innumerable biological applications. The overlap of QDs with the rich chemistry and biology that is characteristic of the peptide arena is an emerging research area. Peptides engineered with appropriate cysteines or histidines have served as ligands for producing water soluble QDs as well as for tagging protein ligands and biosensors to QD surfaces. Incorporation of cell‐penetrating peptides on QD surfaces has allowed for the translocation of functionalized QDs into cells for intracellular imaging applications. QDs containing fluorescently labeled peptide substrates have shown utility in the development of novel protease assays. Moreover, QDs‐labeled peptides that serve as ligands for cellular receptors provide an alternative to antibody mediated imaging at the whole‐cell and single molecule level to study receptor distribution and trafficking. This review highlights the overlap between QD and peptide chemistry and speculates on future research directions. © 2006 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 88: 325‐339, 2007. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Journal
Biopolymers – Wiley
Published: Jan 1, 2007
Keywords: quantum dot; peptide; conjugate; imaging