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Quantitation of resistance of leukemic cells to cytosine arabinoside from BrdUrd/DNA bivariate histograms

Quantitation of resistance of leukemic cells to cytosine arabinoside from BrdUrd/DNA bivariate... The cytotoxicity of ara‐C derives from an inhibition of DNA synthesis after incorporation of ara‐CTP into DNA. The rate of DNA synthesis can be determined from the amount of bromodeoxy‐uridine (BrdUrd) incorporated into cells after a short exposure to BrdUrd. We developed a computer program to quantify the inhibition of the rate of DNA synthesis by analysis of the distribution of BrdUrd/ DNA. Inhibition was evaluated in ara‐C‐sensitive and resistant cells after incubation with different doses of ara‐C. An index of resistance to ara‐C (RI) was expressed as the ratio of the amount of BrdUrd incorporated into S phase cells incubated with ara‐C to that incorporated in the absence of ara‐C. In the ara‐C‐sensitive and resistant HL60 cells, a linear relationship between RI and log ara‐C concentration was observed. Small numbers of slightly resistant cells in mixtures of ara‐C‐sensitive and resistant cells could be determined using this method, making it suitable for clinical use to test the resistance of leukemic cells to ara‐C. © 1992 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cytometry Part A Wiley

Quantitation of resistance of leukemic cells to cytosine arabinoside from BrdUrd/DNA bivariate histograms

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References (29)

Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1552-4922
eISSN
1552-4930
DOI
10.1002/cyto.990130709
pmid
1451603
Publisher site
See Article on Publisher Site

Abstract

The cytotoxicity of ara‐C derives from an inhibition of DNA synthesis after incorporation of ara‐CTP into DNA. The rate of DNA synthesis can be determined from the amount of bromodeoxy‐uridine (BrdUrd) incorporated into cells after a short exposure to BrdUrd. We developed a computer program to quantify the inhibition of the rate of DNA synthesis by analysis of the distribution of BrdUrd/ DNA. Inhibition was evaluated in ara‐C‐sensitive and resistant cells after incubation with different doses of ara‐C. An index of resistance to ara‐C (RI) was expressed as the ratio of the amount of BrdUrd incorporated into S phase cells incubated with ara‐C to that incorporated in the absence of ara‐C. In the ara‐C‐sensitive and resistant HL60 cells, a linear relationship between RI and log ara‐C concentration was observed. Small numbers of slightly resistant cells in mixtures of ara‐C‐sensitive and resistant cells could be determined using this method, making it suitable for clinical use to test the resistance of leukemic cells to ara‐C. © 1992 Wiley‐Liss, Inc.

Journal

Cytometry Part AWiley

Published: Jan 1, 1992

Keywords: ; ; ; ;

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