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J. Nam, C. Thaxtin, C. Mirkin, Becky Kusko (2003)
Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of ProteinsScience, 301
K. Ranade, Mau-Song Chang, C. Ting, D. Pei, C. Hsiao, M. Olivier, Robert Pesich, J. Hebert, Yii-Derr Chen, V. Dzau, D. Curb, R. Olshen, N. Risch, D. Cox, D. Botstein (2001)
High-throughput genotyping with single nucleotide polymorphisms.Genome research, 11 7
Jian-Bing Fan, M. Chee, K. Gunderson (2006)
Highly parallel genomic assaysNature Reviews Genetics, 7
Takaaki Kojima, Yoshiaki Takei, M. Ohtsuka, Y. Kawarasaki, T. Yamane, H. Nakano (2005)
PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targetsNucleic Acids Research, 33
Hebai Shen, Min Hu, Youbao Wang, H. Zhou (2005)
Polymerase chain reaction of nanoparticle-bound primersBiophysical Chemistry, 115
E. Marshall (1997)
The Hunting of the SNPScience, 278
Meiju Ji, P. Hou, Song Li, N. He, Zuhong Lu (2004)
Microarray-based method for genotyping of functional single nucleotide polymorphisms using dual-color fluorescence hybridization.Mutation research, 548 1-2
X. Zhao, Rovelyn Tapec-Dytioco, Kemin Wang, W. Tan (2003)
Collection of trace amounts of DNA/mRNA molecules using genomagnetic nanocapturers.Analytical chemistry, 75 14
Kohei Maruyama, H. Takeyama, Etsuo Nemoto, Tsuyoshi Tanaka, Kiyoshi Yoda, T. Matsunaga (2004)
Single nucleotide polymorphism detection in aldehyde dehydrogenase 2 (ALDH2) gene using bacterial magnetic particles based on dissociation curve analysisBiotechnology and Bioengineering, 87
Zhifei Wang, Huishi Guo, Yi-Qing Yu, N. He (2006)
Synthesis and characterization of a novel magnetic carrier with its composition of Fe3O4/carbon using hydrothermal reactionJournal of Magnetism and Magnetic Materials, 302
E. Pennisi (1998)
A Closer Look at SNPs Suggests DifficultiesScience, 281
E. Marshall (1997)
'Playing Chicken' Over Gene MarkersScience, 278
A novel approach for the genotyping of single nucleotide polymorphisms (SNPs) based on solidphase PCR on magnetic nanoparticles (MNPs) is described. PCR products were amplified directly on MNPs. The genotypes of a given SNP were differentiated by hybridization with a pair of allele‐specific probes labeled with dual‐color fluorescence (Cy3, Cy5). The results were analyzed by scanning the microarray printed with the denatured fluorescent probes on an unmodified glass slide. Electrophoresis analysis indicated that PCR could proceed successfully when MNPs‐bound primers were used. Furthermore, nine different samples were genotyped and their fluorescent signals were quantified. Genotyping results showed that three genotypes for the locus were very easily discriminated. The fluorescent ratios (match probe:mismatch probe signal) of homozygous samples were over 9.3, whereas heterozygous samples had ratios near 1.0. Without any purification and concentration of PCR products, this new MNP‐PCR based genotyping assay potentially provides a rapid, labor‐saving method for genotyping of a large number of individuals.
Biotechnology Journal – Wiley
Published: Apr 1, 2007
Keywords: ; ; ;
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