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R McCabe, H Feister, JM Hock, JP Bidwell (1996)
Nuclear matrix and intermediate filament proteins of a human osteosarcoma tumor, 75
J. Juenke, J. Holden (1993)
The distribution of DNA topoisomerase II isoforms in differentiated adult mouse tissues.Biochimica et biophysica acta, 1216 2
Masahiko Watanabe, K. Tsutsui, K. Tsutsui, Y. Inoue (1994)
Differential expressions of the topoisomerase IIα and IIβ mRNAs in developing rat brainNeuroscience Research, 19
S. Dworetzky, E. Fey, S. Penman, J. Lian, J. Stein, G. Stein (1990)
Progressive changes in the protein composition of the nuclear matrix during rat osteoblast differentiation.Proceedings of the National Academy of Sciences of the United States of America, 87
J. Mirkovitch, M. Mirault, U. Laemmli (1984)
Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffoldCell, 39
J. Bidwell, E. Fey, A. Wijnen, S. Penman, J. Stein, J. Lian, G. Stein (1994)
Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells.Cancer research, 54 1
G. Capranico, S. Tinelli, C. Austin, M. Fisher, F. Zunino (1992)
Different patterns of gene expression of topoisomerase II isoforms in differentiated tissues during murine development.Biochimica et biophysica acta, 1132 1
J. Nickerson, S. Penman (1992)
Localization of nuclear matrix core filament proteins at interphase and mitosis.Cell biology international reports, 16 8
R. Nissenson, Z. Huang, E. Blind, D. Shoback (1993)
Structure and function of the receptor for parathyroid hormone and parathyroid hormone-related protein.Receptor, 3 3
J. Bidwell, A. Wijnen, C. Banerjee, E. Fey, H. Merriman, S. Penman, J. Stein, J. Lian, G. Stein (1994)
Parathyroid-responsive modifications in the nuclear matrix of ROS 17/2.8 rat osteosarcoma cells.Endocrinology, 134 4
K. Carter, D. Bowman, W. Carrington, K. Fogarty, J. Mcneil, F. Fay, J. Lawrence (1993)
A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus.Science, 259 5099
H. Merriman, A. Wijnen, S. Hiebert, J. Bidwell, E. Fey, J. Lian, J. Stein, G. Stein (1995)
The tissue-specific nuclear matrix protein, NMP-2, is a member of the AML/CBF/PEBP2/runt domain transcription factor family: interactions with the osteocalcin gene promoter.Biochemistry, 34 40
A. Lomri, P. Marie (1990)
Changes in cytoskeletal proteins in response to parathyroid hormone and 1,25-dihydroxyvitamin D in human osteoblastic cells.Bone and mineral, 10 1
Margarete Heck, Walter HittelmanT, W. Earnshaw (1988)
Differential expression of DNA topoisomerases I and II during the eukaryotic cell cycle.Proceedings of the National Academy of Sciences of the United States of America, 85 4
H Long, J Onyia, J Hulman, J Bidwel, JM Hock (1995)
Molecular characterizations of primary spongiosa calls from distal femurs of young rats, 10
J Mirkovitch, M‐E Mirault, UK Laemmli (1984)
Organization of the higher order chromatin loop: Specific DNA attachment attachment sites on nuclear scaffold, 39
E. Fey, K. Wan, S. Penman (1984)
Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three-dimensional organization and protein compositionThe Journal of Cell Biology, 98
D. Compton, Chenghua Luo (1995)
Mutation of the predicted p34cdc2 phosphorylation sites in NuMA impair the assembly of the mitotic spindle and block mitosis.Journal of cell science, 108 ( Pt 2)
Carol Webb, Kenton Eneff, Fred Drake (1993)
A topoisomerase II-like protein is part of an inducible DNA-binding protein complex that binds 5' of an immunoglobulin promoter.Nucleic acids research, 21 18
R. Getzenberg, K. Pienta, D. Coffey (1990)
The tissue matrix: cell dynamics and hormone action.Endocrine reviews, 11 3
D. Wigley (1995)
Structure and mechanism of DNA topoisomerases.Annual review of biophysics and biomolecular structure, 24
C. Zeng, D. He, B. Brinkley (1994)
Localization of NuMA protein isoforms in the nuclear matrix of mammalian cells.Cell motility and the cytoskeleton, 29 2
J. Egan, G. Gronowicz, G. Rodan (1991)
Parathyroid hormone promotes the disassembly of cytoskeletal actin and myosin in cultured osteoblastic cells: Mediation by cyclic AMPJournal of Cellular Biochemistry, 45
S. Razin, I. Gromova (1995)
The channels model of nuclear matrix structureBioEssays, 17
Ronald Berezney, D. Coffey (1975)
Nuclear protein matrix: association with newly synthesized DNA.Science, 189 4199
N. Partridge, S. Bloch, A. Pearman (1994)
Signal transduction pathways mediating parathyroid hormone regulation of osteoblastic gene expressionJournal of Cellular Biochemistry, 55
J. Bodnar, G. Jones, C. Ellis (1989)
The domain model for eukaryotic DNA organization. 2: A molecular basis for constraints on development and evolution.Journal of theoretical biology, 137 3
G. Giaccone (1994)
DNA topoisomerases and topoisomerase inhibitors.Pathologie-biologie, 42 4
K. Nakagomi, Y. Kohwi, L. Dickinson, T. Kohwi-Shigematsu (1994)
A novel DNA-binding motif in the nuclear matrix attachment DNA-binding protein SATB1Molecular and Cellular Biology, 14
J. Egan, G. Gronowicz, G. Rodan (1991)
Cell density‐dependent decrease in cytoskeletal actin and myosin in cultured osteoblastic cells: Correlation with cyclic AMP changesJournal of Cellular Biochemistry, 45
(1987)
Current Protocols in Molecular Biology
R. Berezney (1991)
The nuclear matrix: A heuristic model for investigating genomic organization and function in the cell nucleusJournal of Cellular Biochemistry, 47
Parathyroid hormone (PTH) alters osteoblast morphology. How these changes in cell shape modify nuclear structure and ultimately gene expression is not known. Chronic exposure to rat PTH (1–34) [10 nM] attenuated the expression of 200, 190, and 160 kD proteins in the nuclear matrix‐intermediate filament subfraction of the rat osteosarcoma cells, ROS 17/2.8 [Bidwell et al. (1994b): Endocrinology 134:1738–1744]. Here, we determined that these same PTH‐responsive proteins were expressed in rat metaphyseal osteoblasts. We identified the 200 kD protein as a non‐muscle myosin. Although the molecular weights, subcellular distribution, and half‐lives of the 190 and 160 kD proteins were similar to topoisomerase II‐α and ‐β, nuclear matrix enzymes that mediate DNA topology, the 190 and 160 kD proteins did not interact with topoisomerase antibodies. Nevertheless, the expression of topoisomerase II‐α, and NuMA, a component of the nuclear core filaments, was also regulated by PTH in the osteosarcoma cells. The 190 kD protein was selectively expressed in bone cells as it was not observed in OK opossum kidney cells, H4 hepatoma cells, or NIH3T3 cells. PTH attenuated mRNA expression of the PTH receptor in our cell preparations. These results demonstrate that PTH selectively alters the expression of osteoblast membrane, cytoskeletal, and nucleoskeletal proteins. Topoisomerase II‐α, NuMA, and the 190 and 160 kD proteins may direct the nuclear PTH signalling pathways to the target genes and play a structural role in osteoblast gene expression. © 1996 Wiley‐Liss, Inc.
Journal of Cellular Biochemistry – Wiley
Published: Jan 1, 1996
Keywords: ; ; ; ;
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