Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Oxidation of glucose by mouse peritoneal macrophages: A comparison of suspensions and monolayers

Oxidation of glucose by mouse peritoneal macrophages: A comparison of suspensions and monolayers 10.1002/jcp.1041050202.abs Macrophages, when maintained in vitro, take up glucose from the medium and oxidize it to CO2. The rate of oxidation of glucose varies considerably, depending on the physical state of the cell preparation. Cells in suspension oxidize glucose at a level six‐fold that of cells in monolayers. The differences cannot be attributed to change in the rates of transport of glucose. On the other hand, an increse in intracellular glycogen (about three‐fold) and free glucose plus glucose‐6‐P (many‐fold) was found in the cells prepared as monolayers. During subsequent incubation with glucose‐14C, this could be the cause of an isotope dilution effect and could explain the lower production of 14CO2 by the adherent cells. Since oxidation of glucose‐1‐14C to 14CO2 is used by many investigators to indicate the functional state of macrophages, we suggest close attention be paid to the system used, i.e., monolayers vs. suspensions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Physiology Wiley

Oxidation of glucose by mouse peritoneal macrophages: A comparison of suspensions and monolayers

Download PDF
6 pages

Loading next page...
 
/lp/wiley/oxidation-of-glucose-by-mouse-peritoneal-macrophages-a-comparison-of-k00YJ9kVq5

References (17)

Publisher
Wiley
Copyright
Copyright © 1980 Wiley‐Liss, Inc.
ISSN
0021-9541
eISSN
1097-4652
DOI
10.1002/jcp.1041050202
pmid
6780573
Publisher site
See Article on Publisher Site

Abstract

10.1002/jcp.1041050202.abs Macrophages, when maintained in vitro, take up glucose from the medium and oxidize it to CO2. The rate of oxidation of glucose varies considerably, depending on the physical state of the cell preparation. Cells in suspension oxidize glucose at a level six‐fold that of cells in monolayers. The differences cannot be attributed to change in the rates of transport of glucose. On the other hand, an increse in intracellular glycogen (about three‐fold) and free glucose plus glucose‐6‐P (many‐fold) was found in the cells prepared as monolayers. During subsequent incubation with glucose‐14C, this could be the cause of an isotope dilution effect and could explain the lower production of 14CO2 by the adherent cells. Since oxidation of glucose‐1‐14C to 14CO2 is used by many investigators to indicate the functional state of macrophages, we suggest close attention be paid to the system used, i.e., monolayers vs. suspensions.

Journal

Journal of Cellular Physiology – Wiley

Published: Nov 1, 1980

There are no references for this article.