Access the full text.
Sign up today, get DeepDyve free for 14 days.
R. Kurnik, Audrey Yu, G. Blank, Arlene Burton, David Smith, A. Athalye, R. Reis (1995)
Buffer exchange using size exclusion chromatography, countercurrent dialysis, and tangential flow filtration: Models, development, and industrial applicationBiotechnology and Bioengineering, 45
James Falsey, Manat Renil, Steven Park, Shijun Li, Kit Lam (2001)
Peptide and small molecule microarray for high throughput cell adhesion and functional assays.Bioconjugate chemistry, 12 3
L. Zeman, A. Zydney (1996)
Microfiltration and Ultrafiltration: Principles and Applications
A. Denizli, Erhan Pişkin (2001)
Dye-ligand affinity systems.Journal of biochemical and biophysical methods, 49 1-3
P. Ng, J. Lundblad, G. Mitra (1976)
Optimization of Solute Separation by DiafiltrationSeparation Science and Technology, 11
E. Pidcock, G. Moore (2001)
Structural characteristics of protein binding sites for calcium and lanthanide ionsJBIC Journal of Biological Inorganic Chemistry, 6
R. Madsen (2001)
Design of sanitary and sterile UF- and diafiltration plantsSeparation and Purification Technology, 22
P. Ng, J. Lundblad, G. Mitra (1976)
透析ろ過(diafiltration)による溶質の最適分離条件, 11
Optimal protein concentration for performing the diafiltration as a function of the wall concentration for several values of the equilibrium binding constant
E. Ueda, P. Gout, L. Morganti (2003)
Current and prospective applications of metal ion-protein binding.Journal of chromatography. A, 988 1
O. Yamauchi, A. Odani, S. Hirota (2001)
Metal Ion-Assisted Weak Interactions Involving Biological Molecules. From Small Complexes to MetalloproteinsBulletin of the Chemical Society of Japan, 74
W. Bowen, R. Nigmatullin (2002)
MEMBRANE-ASSISTED CHIRAL RESOLUTION OF PHARMACEUTICALS: IBUPROFEN SEPARATION BY ULTRAFILTRATION USING BOVINE SERUM ALBUMIN AS CHIRAL SELECTORSeparation Science and Technology, 37
Jonathan Romero, A. Zydney (2001)
CHIRAL SEPARATIONS USING ULTRAFILTRATION WITH A STEREOSELECTIVE BINDING AGENTSeparation Science and Technology, 36
Ultrafiltration and diafiltration processes are used extensively for removal of a variety of small impurities from biological products. There has, however, been no experimental or theoretical analysis of the effects of impurity— product binding on the rate of impurity removal during these processes. Model calculations were performed to account for the effects of equilibrium binding between a small impurity and a large (retained) product on impurity clearance. Experiments were performed using D‐tryptophan and bovine serum albumin as a model system. The results clearly demonstrate that binding interactions can dramatically reduce the rate of small impurity removal, leading to large increases in the required number of diavolumes. The optimal product concentration for performing the diafiltration shifts to lower product concentrations in the presence of strong binding interactions. Approximate analytical expressions for the impurity removal were developed which can provide a guide for the design and optimization of industrial ultrafiltration/diafiltration processes. © 2004 Wiley Periodicals, Inc.
Biotechnology and Bioengineering – Wiley
Published: Aug 5, 2004
Keywords: impurity removal; ultrafiltration; diafiltration; clearance; binding interactions; buffer exchange
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.