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In this issue: Biotechnology Journal 2/2010

In this issue: Biotechnology Journal 2/2010 Biotechnology Biotechnol. J. 2010, 5, 136 Journal In this issue Barley, a green ligand factory Erlendsson et al, Biotechnol. J. 2009, 5, 163–171. Growth factors are commonly used as cell culture supplements and might gain even more importance in stem cell research, tissue engineering and regenerative medicine or as human therapeutics. Recombinant growth factors are commercially produced in bacteria, yeast, insect and mammalian cells. While these systems are efficient, there is still a need for serum and endotoxin free production systems. Therefore, researchers from Island and co-workers produced biologically active re- combinant human Flt3 ligand in transgenic barley seeds. This ligand can then be purified with a tag using immobilized metal ion affinity chromatography (IMAC). The Flt3 is posttranslationally modified and exhibits comparable biological activity to commercial Flt3 ligand. This study demonstrates that plant molecular farming © PhotoDisc is a viable approach for the bioproduction of human-derived growth factors. .. 163 http://dx.doi.org/10.1002/biot.200900111 Transgenic crop safety Herman et al, Biotechnol. J. 2009, 5, 172–182. To evalutate the safety of transgenic crops one can analyse the composition of the trans- genic crop and compare it to the parent plant or seed. If the composition of nutrients is found to be equivalent to that of non-transgenic variant that is considered safe, then fur- ther safety assessment of the transgenic crop can focus solely on the intended modifica- tion, like the expression of a transgenic protein. Statistical methods that are used in clin- ical medicine to compare new generic drugs with brand-name drugs can then be applied to evaluate the equivalence. However, commonly used equivalence limits are shown to be a poor model for comparing transgenic crops with an array of reference crop vari- eties. Researchers from Dow AgroSciences LLC (USA) suggest an alternate model applied to corn, cotton and soybean seed samples. ...................................................... 172 http://dx.doi.org/10.1002/biot.200900217 Advanced biofuels from microbes Peralta-Yahya and Keasling, Biotechnol. J. 2009, 5, 147–162. The climate change and energy security are the major challenges of current times. Bio- fuels produced from renewable resourses are a cost-effective alternative. Ethanol produced by microorganisms is currently the major biofuel in the transportation sector. However, ethanol’s corrosivity and hygroscopicity make it incompatible with existing fuel storage and distribution infrastructure and limits its economic use. Advanced biofuels, such as long chain alcohols and isoprenoid- and fatty acid-based biofuels, have physical proper- ties that more closely resemble petroleum-derived fuels. Therefore, they are attractive candidates for the replacement of petroleum-derived fuels. Authors from the University of California, Berkeley, review recent developments in the engineering of metabolic path- ways for the production of advanced biofuels by microorganisms, most importantly Escherichia coli and Saccharomyces cerevisiae. .................................................................. 147 © PhotoDisc/Getty Images http://dx.doi.org/10.1002/biot.200900220 136 © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim © PhotoDisc http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Journal Wiley

In this issue: Biotechnology Journal 2/2010

Biotechnology Journal , Volume 5 (2) – Feb 1, 2010
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Publisher
Wiley
Copyright
Copyright © 2010 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1860-6768
eISSN
1860-7314
DOI
10.1002/biot.201090007
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Abstract

Biotechnology Biotechnol. J. 2010, 5, 136 Journal In this issue Barley, a green ligand factory Erlendsson et al, Biotechnol. J. 2009, 5, 163–171. Growth factors are commonly used as cell culture supplements and might gain even more importance in stem cell research, tissue engineering and regenerative medicine or as human therapeutics. Recombinant growth factors are commercially produced in bacteria, yeast, insect and mammalian cells. While these systems are efficient, there is still a need for serum and endotoxin free production systems. Therefore, researchers from Island and co-workers produced biologically active re- combinant human Flt3 ligand in transgenic barley seeds. This ligand can then be purified with a tag using immobilized metal ion affinity chromatography (IMAC). The Flt3 is posttranslationally modified and exhibits comparable biological activity to commercial Flt3 ligand. This study demonstrates that plant molecular farming © PhotoDisc is a viable approach for the bioproduction of human-derived growth factors. .. 163 http://dx.doi.org/10.1002/biot.200900111 Transgenic crop safety Herman et al, Biotechnol. J. 2009, 5, 172–182. To evalutate the safety of transgenic crops one can analyse the composition of the trans- genic crop and compare it to the parent plant or seed. If the composition of nutrients is found to be equivalent to that of non-transgenic variant that is considered safe, then fur- ther safety assessment of the transgenic crop can focus solely on the intended modifica- tion, like the expression of a transgenic protein. Statistical methods that are used in clin- ical medicine to compare new generic drugs with brand-name drugs can then be applied to evaluate the equivalence. However, commonly used equivalence limits are shown to be a poor model for comparing transgenic crops with an array of reference crop vari- eties. Researchers from Dow AgroSciences LLC (USA) suggest an alternate model applied to corn, cotton and soybean seed samples. ...................................................... 172 http://dx.doi.org/10.1002/biot.200900217 Advanced biofuels from microbes Peralta-Yahya and Keasling, Biotechnol. J. 2009, 5, 147–162. The climate change and energy security are the major challenges of current times. Bio- fuels produced from renewable resourses are a cost-effective alternative. Ethanol produced by microorganisms is currently the major biofuel in the transportation sector. However, ethanol’s corrosivity and hygroscopicity make it incompatible with existing fuel storage and distribution infrastructure and limits its economic use. Advanced biofuels, such as long chain alcohols and isoprenoid- and fatty acid-based biofuels, have physical proper- ties that more closely resemble petroleum-derived fuels. Therefore, they are attractive candidates for the replacement of petroleum-derived fuels. Authors from the University of California, Berkeley, review recent developments in the engineering of metabolic path- ways for the production of advanced biofuels by microorganisms, most importantly Escherichia coli and Saccharomyces cerevisiae. .................................................................. 147 © PhotoDisc/Getty Images http://dx.doi.org/10.1002/biot.200900220 136 © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim © PhotoDisc

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Biotechnology Journal – Wiley

Published: Feb 1, 2010

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