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- Publisher
- Wiley
- Copyright
- Copyright © 2009 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1860-6768
- eISSN
- 1860-7314
- DOI
- 10.1002/biot.200900016
- pmid
- 19557797
- Publisher site
- See Article on Publisher Site
Abstract
We report highly sensitive bioluminescence resonance energy transfer (BRET) assays with optimized donor/acceptor couples. We combined the energy donors Renilla luciferase (Rluc) and the Rluc8 variant with the energy acceptors yellow fluorescent protein, the YPet variant and the Renilla green fluorescent protein (RGFP). Different donor/acceptor couples were tested in well‐established assays measuring ligand‐induced β‐arrestin (βARR) intramolecular rearrangements and recruitment to G protein‐coupled receptors. We show increased sensitivity with Rluc8/YPet and Rluc8/RGFP couples and measured previously undetectable BRET signals. These tools improve existing βARR assays and offer new options for the development of future BRET assays.
Journal
Biotechnology Journal – Wiley
Published: Sep 1, 2009
Keywords: ; ; ; ;