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A DQβ locus‐specific oligonucleotide probe is used to identify DQβ alleles present in six insulin‐dependent diabetes mellitus (IDDM) multiplex families. Results of genotyping using this technique agree with those from cDNA probes and are often clearer. Allele‐specific oligonucleotide probes for the DRβ locus are used to identify subtypes (Dw4 and Dw14) of the DR4 specificity in five IDDM multiplex families. This distinction cannot be made with available cDNA probes.
Genetic Epidemiology – Wiley
Published: Jan 1, 1989
Keywords: HLA class II alleles; HLA‐DR4 subtypes; HLA DQβ genotypes; IDDM; RFLP analysis; molecular genotyping techniques; Dw4‐83 oligonucleotide; Dw14‐68 oligonucleotide; DQβ‐5 oligonucleotide; locus‐specific probes; allele‐specific probes
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