Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/fluorogenic-substrates-for-lipases-esterases-and-acylases-using-a-tim-EBpyoyoY4i
References (40)
-
G. Guilbault, J. Hieserman (1969)
Fluorometric substrate for sulfatase and lipase.Analytical chemistry, 41 14
-
E. Leroy, Nicolas Bensel, J. Reymond (2003)
A low background high-throughput screening (HTS) fluorescence assay for lipases and esterases using acyloxymethylethers of umbelliferone.Bioorganic & medicinal chemistry letters, 13 13
-
Yongzheng Yang, P. Babiak, J. Reymond (2006)
Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions.Organic & biomolecular chemistry, 4 9
-
M. Reetz (2001)
Combinatorial and Evolution-Based Methods in the Creation of Enantioselective Catalysts.Angewandte Chemie, 40 2
-
R. Schmid, R. Verger (1998)
Lipases: Interfacial Enzymes with Attractive Applications.Angewandte Chemie, 37 12
-
Jacob Kofoed, T. Darbre, J. Reymond (2006)
Artificial aldolases from peptide dendrimer combinatorial libraries.Organic & biomolecular chemistry, 4 17
-
B. Bicalho, Lu Chen, Johann Grognux, J. Reymond, A. Marsaioli (2004)
Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenasesJournal of the Brazilian Chemical Society, 15
-
B. Norledge, A. Lambeir, R. Abagyan, A. Rottmann, A. Fernández, V. Filimonov, M. Peter, R. Wierenga (2001)
Modeling, mutagenesis, and structural studies on the fully conserved phosphate‐binding loop (Loop 8) of triosephosphate isomerase: Toward a new substrate specificityProteins: Structure, 42
-
Johann Grognux, D. Wahler, Erich Nyfeler, J. Reymond (2004)
Universal chromogenic substrates for lipases and esterasesTetrahedron-asymmetry, 15
-
G. Klein, J. Reymond (1998)
An enantioselective fluorimetric assay for alcohol dehydrogenases using albumin-catalyzed beta-elimination of umbelliferone.Bioorganic & medicinal chemistry letters, 8 9
-
A. Archelas, R. Furstoss (2001)
Synthetic applications of epoxide hydrolases.Current opinion in chemical biology, 5 2
-
Marlen Schmidt, U. Bornscheuer (2005)
High-throughput assays for lipases and esterases.Biomolecular engineering, 22 1-3
-
J. Goddard, J. Reymond (2004)
Recent advances in enzyme assays.Trends in biotechnology, 22 7
-
O. Khersonsky, Dan Tawfik (2006)
Chromogenic and Fluorogenic Assays for the Lactonase Activity of Serum ParaoxonasesChemBioChem, 7
-
Jacob Kofoed, T. Darbre, J. Reymond (2006)
Dual mechanism of zinc-proline catalyzed aldol reactions in water.Chemical communications, 14
-
D. Wahler, J. Reymond (2002)
The adrenaline test for enzymes.Angewandte Chemie, 41 7
-
Erich Nyfeler, Johann Grognux, D. Wahler, J. Reymond (2003)
A Sensitive and Selective High-Throughput Screening Fluorescence Assay for Lipases and EsterasesHelvetica Chimica Acta, 86
-
Fabrizio Badalassi, Hong-Khanh Nguyen, P. Crotti, J. Reymond (2002)
A Selective HIV-Protease Assay Based on a Chromogenic Amino AcidHelvetica Chimica Acta, 85
-
J. Goddard, J. Reymond (2004)
Enzyme assays for high-throughput screening.Current opinion in biotechnology, 15 4
-
Hening Lin, V. Cornish (2002)
Screening and selection methods for large-scale analysis of protein function.Angewandte Chemie, 41 23
-
G. Klein, J. Reymond (1999)
Enantioselective Fluorogenic Assay of Acetate Hydrolysis for Detecting Lipase Catalytic AntibodiesHelvetica Chimica Acta, 82
-
Fabrizio Badalassi, D. Wahler, G. Klein, P. Crotti, J. Reymond (2000)
A Versatile Periodate-Coupled Fluorogenic Assay for Hydrolytic EnzymesAngewandte Chemie, 39
-
Yongzheng Yang, P. Babiak, J. Reymond (2006)
New Monofunctionalized Fluorescein Derivatives for the Efficient High-Throughput Screening of Lipases and Esterases in Aqueous MediaHelvetica Chimica Acta, 89
-
D. Wahler, O. Boujard, F. Lefèvre, J. Reymond (2004)
Adrenaline profiling of lipases and esterases with 1,2-diol and carbohydrate acetatesTetrahedron, 60
-
E. Leroy, Nicolas Bensel, J. Reymond (2003)
Fluorogenic Cyanohydrin Esters as Chiral Probes for Esterase and Lipase ActivityAdvanced Synthesis & Catalysis, 345
-
D. Lagarde, Hong-Khanh Nguyen, G. Ravot, D. Wahler, J. Reymond, G. Hills, T. Veit, F. Lefèvre (2002)
High-throughput screening of thermostable esterases for industrial bioconversionsOrganic Process Research & Development, 6
-
M. Reetz (2002)
Lipases as practical biocatalysts.Current opinion in chemical biology, 6 2
-
R. Eisenthal, M. Danson (1992)
Enzyme assays : a practical approach -
S. Gul, S. Sreedharan, K. Brocklehurst (1998)
Enzyme Assays: Essential Data -
E. González‐García, Johann Grognux, D. Wahler, J. Reymond (2003)
Synthesis and Evaluation of Chromogenic and Fluorogenic Analogs of Glycerol for Enzyme AssaysHelvetica Chimica Acta, 86
-
M. Chini, P. Crotti, Lucilla Favero, F. Macchia, M. Pineschi (1994)
Lanthanide(III) trifluoromethanesulfonates as extraordinarily effective new catalysts for the aminolysis of 1,2-epoxidesTetrahedron Letters, 35
-
J. Reymond (2004)
Spectrophotometric Enzyme Assays for High-Throughput ScreeningFood Technology and Biotechnology, 42
-
D. Wahler, J. Reymond (2001)
High-throughput screening for biocatalysts.Current opinion in biotechnology, 12 6
-
Fabrizio Badalassi, G. Klein, P. Crotti, J. Reymond (2004)
Fluorescence Assay and Screening of Epoxide Opening by NucleophilesEuropean Journal of Organic Chemistry, 2004
-
D. Gilham, R. Lehner (2005)
Techniques to measure lipase and esterase activity in vitro.Methods, 36 2
-
D. Wahler, J. Reymond (2001)
Novel methods for biocatalyst screening.Current opinion in chemical biology, 5 2
-
Johann Grognux, J. Reymond (2006)
A red-fluorescent substrate microarray for lipase fingerprinting.Molecular bioSystems, 2 10
-
P. Babiak, J. Reymond (2005)
A high-throughput, low-volume enzyme assay on solid support.Analytical chemistry, 77 2
-
W. Alkema, E. Vries, R. Floris, D. Janssen (2003)
Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of beta-lactam antibiotics.European journal of biochemistry, 270 18
-
R. Sicard, Lu Chen, A. Marsaioli, J. Reymond (2005)
A Fluorescence-Based Assay for Baeyer - Villiger Monooxygenases, Hydroxylases and LactonasesAdvanced Synthesis & Catalysis, 347
- Publisher
- Wiley
- Copyright
- Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1860-6768
- eISSN
- 1860-7314
- DOI
- 10.1002/biot.200600181
- pmid
- 17219461
- Publisher site
- See Article on Publisher Site
Abstract
3‐Acyloxyl‐2‐oxopropyl ethers of umbelliferone were investigated as new fluorogenic substrates for lipases and esterases. The aliphatic primary alcohol‐leaving group released the fluorescent product umbelliferone by an enolization/β‐elimination reaction similar to the triose phosphate isomerase (TIM) reaction. A similarly designed phenylacetamide provided a fluorescent probe for penicillin G acylase, whereby the enolization/β‐elimination sequence from the intermediate aminoketone was very fast and spontaneous even under acidic conditions. The corresponding epoxyketone was not fluorogenic with epoxide hydrolases (EH). These substrates represent periodate‐free Clips‐o™ substrates.
Journal
Biotechnology Journal – Wiley
Published: Feb 1, 2007
Keywords: ; ; ; ;