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Effects of buffering intracellular free calcium on neutrophil migration through three‐dimensional matrices

Effects of buffering intracellular free calcium on neutrophil migration through three‐dimensional... 10.1002/(SICI)1097-4652(199705)171:2<168::AID-JCP7>3.3.CO;2-I Repeated transient increases in intracellular free calcium levels ((Ca2+)i) are required for polymorphonuclear neutrophil migration on two‐dimensional surfaces coated with fibronectin or vitronectin. Cells in which (Ca2+)iis buffered with quin2 become stuck on these substrates. Neutrophils migrating through the extracellular matrix in vivo encounter these and other substrates in a three‐dimensional architecture that may alter the spatial distribution of adhesion receptors in contact with the matrix. In this study, we used fluorescence confocal microscopy to obtain moving three‐dimensional images of neutrophils migrating through a biological tissue (human amnion) in the presence and absence of (Ca2+)i‐buffering with quin2. In the absence of buffering, (Ca2+)itransients similar to those seen in cells migrating in two‐dimensions were observed. (Ca2+)i‐buffered neutrophils were able to migrate into the matrix, but they became attached firmly to the substrate at the rear of the cell, resulting in a drastically elongated morphology. Immunofluorescence revealed that neutrophils adhered to regions of the matrix that contained fibronectin. RGD‐containing peptides and antibodies that block integrin adhesion receptors for fibronectin and vitronectin were able to rescue the migration of quin2‐treated cells through three‐dimensional gels containing fibronectin and vitronectin. These data show that neutrophils migrating throughphysiologically relevant, three‐dimensional matrices undergo repetitive increases in (Ca2+)ithat are required for integrin‐mediated detachment from the matrix. J. Cell. Physiol. 171:168–178, 1997. © 1997 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Physiology Wiley

Effects of buffering intracellular free calcium on neutrophil migration through three‐dimensional matrices

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References (46)

Publisher
Wiley
Copyright
Copyright © 1997 Wiley‐Liss, Inc.
ISSN
0021-9541
eISSN
1097-4652
DOI
10.1002/(SICI)1097-4652(199705)171:2<168::AID-JCP7>3.0.CO;2-M
pmid
9130464
Publisher site
See Article on Publisher Site

Abstract

10.1002/(SICI)1097-4652(199705)171:2<168::AID-JCP7>3.3.CO;2-I Repeated transient increases in intracellular free calcium levels ((Ca2+)i) are required for polymorphonuclear neutrophil migration on two‐dimensional surfaces coated with fibronectin or vitronectin. Cells in which (Ca2+)iis buffered with quin2 become stuck on these substrates. Neutrophils migrating through the extracellular matrix in vivo encounter these and other substrates in a three‐dimensional architecture that may alter the spatial distribution of adhesion receptors in contact with the matrix. In this study, we used fluorescence confocal microscopy to obtain moving three‐dimensional images of neutrophils migrating through a biological tissue (human amnion) in the presence and absence of (Ca2+)i‐buffering with quin2. In the absence of buffering, (Ca2+)itransients similar to those seen in cells migrating in two‐dimensions were observed. (Ca2+)i‐buffered neutrophils were able to migrate into the matrix, but they became attached firmly to the substrate at the rear of the cell, resulting in a drastically elongated morphology. Immunofluorescence revealed that neutrophils adhered to regions of the matrix that contained fibronectin. RGD‐containing peptides and antibodies that block integrin adhesion receptors for fibronectin and vitronectin were able to rescue the migration of quin2‐treated cells through three‐dimensional gels containing fibronectin and vitronectin. These data show that neutrophils migrating throughphysiologically relevant, three‐dimensional matrices undergo repetitive increases in (Ca2+)ithat are required for integrin‐mediated detachment from the matrix. J. Cell. Physiol. 171:168–178, 1997. © 1997 Wiley‐Liss, Inc.

Journal

Journal of Cellular Physiology – Wiley

Published: May 1, 1997

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