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Different behavior of native and denatured DNA in the interaction with tris(ethylenediamine) co(III) complex ions

Different behavior of native and denatured DNA in the interaction with tris(ethylenediamine)... D i e r e n t Behavior oj Native and Denatured D N A in the Interaction with Tris(ethy1enediamine) Co(I1I) Complex Ions Peculiar variations in the ultraviolet circular dichroism spectra of native DNA in aqueous- alcoholic solutions’ in films a t low degree of relative humidity2 and in solutions of superhelical DNA3 were recently observed and interpreted as an indication of a change in the secondary structure of the macromolecule. More recently, we have found similar changes in t.he circular dichroism spectra of native DNA in aqueous solutions upon binding with [Co(en)$] complex counterions. On the basis of stereochemical considerations as well as of a number of other experimental findings we concluded that in this case the perturbation in the secondary structure of DNA chiefly arises from the accommodation of the octahedral complex molecules in the major groove of the double helix.4 This conclusion is now also supported by the results obtained with denatured DNA. Denatured DNA was obtained by the method of alkaline denaturation5of highly polymerized calf thymus DNA (Worthington Corp., U.S. !. Some modifications were necessary, however, because of the relatively large amount of material required for the present investigation. In each batch, 0.3 ml http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biopolymers Wiley

Different behavior of native and denatured DNA in the interaction with tris(ethylenediamine) co(III) complex ions

Biopolymers , Volume 12 (10) – Oct 1, 1973

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References (9)

Publisher
Wiley
Copyright
Copyright © 1973 John Wiley & Sons, Inc.
ISSN
0006-3525
eISSN
1097-0282
DOI
10.1002/bip.1973.360121020
Publisher site
See Article on Publisher Site

Abstract

D i e r e n t Behavior oj Native and Denatured D N A in the Interaction with Tris(ethy1enediamine) Co(I1I) Complex Ions Peculiar variations in the ultraviolet circular dichroism spectra of native DNA in aqueous- alcoholic solutions’ in films a t low degree of relative humidity2 and in solutions of superhelical DNA3 were recently observed and interpreted as an indication of a change in the secondary structure of the macromolecule. More recently, we have found similar changes in t.he circular dichroism spectra of native DNA in aqueous solutions upon binding with [Co(en)$] complex counterions. On the basis of stereochemical considerations as well as of a number of other experimental findings we concluded that in this case the perturbation in the secondary structure of DNA chiefly arises from the accommodation of the octahedral complex molecules in the major groove of the double helix.4 This conclusion is now also supported by the results obtained with denatured DNA. Denatured DNA was obtained by the method of alkaline denaturation5of highly polymerized calf thymus DNA (Worthington Corp., U.S. !. Some modifications were necessary, however, because of the relatively large amount of material required for the present investigation. In each batch, 0.3 ml

Journal

BiopolymersWiley

Published: Oct 1, 1973

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