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R. Shalgi, T. Raz (1997)
The role of carbohydrate residues in mammalian fertilization.Histology and histopathology, 12 3
D. Veeramachaneni, Mary Smith, N. Ellinwood (1998)
Deficiency of fucosidase results in acrosomal dysgenesis and impaired sperm maturation.Journal of andrology, 19 4
S. Johnson, J. Alhadeff (1991)
Mammalian alpha-L-fucosidases.Comparative biochemistry and physiology. B, Comparative biochemistry, 99 3
D. Busso, D. Cohen, M. Hayashi, M. Kasahara, P. Cuasnicú (2005)
Human testicular protein TPX1/CRISP-2: localization in spermatozoa, fate after capacitation and relevance for gamete interaction.Molecular human reproduction, 11 4
M. Jiménez-Movilla, M. Avilés, M. Gómez-Torres, P. Fernández-Colom, M. Castells, J. Juan, A. Romeu, J. Ballesta (2004)
Carbohydrate analysis of the zona pellucida and cortical granules of human oocytes by means of ultrastructural cytochemistry.Human reproduction, 19 8
G. Andrews-Smith, J. Alhadeff (1982)
Radioimmunoassay determinations of decreased amounts of α-L-fucosidase protein in fucosidosisBiochimica et Biophysica Acta, 715
S. Benoff (1997)
Carbohydrates and fertilization: an overview.Molecular human reproduction, 3 7
J. Alhadeff, S. Khunsook, K. Choowongkomon, T. Baney, V. Heredia, A. Tweedie, B. Bean (1999)
Characterization of human semen alpha-L-fucosidases.Molecular human reproduction, 5 9
(1002)
Molecular Reproduction and Development. DOI
J. Tesarik, J. Tesarik, C. Mendoza, C. Mendoza, Juan-Pablo Ramirez, J. Ramírez, J. Moos, J. Moos (1993)
Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface.Fertility and sterility, 60 2
M. Matsumoto, J. Hirata, N. Hirohashi, M. Hoshi (2002)
Sperm-egg binding mediated by sperm alpha-L-fucosidase in the ascidian, Halocynthia roretzi.Zoological science, 19 1
J. Michalski, A. Klein (1999)
Glycoprotein lysosomal storage disorders: alpha- and beta-mannosidosis, fucosidosis and alpha-N-acetylgalactosaminidase deficiency.Biochimica et biophysica acta, 1455 2-3
M. Herrero, A. Mandal, L. Digilio, S. Coonrod, B. Maier, J. Herr (2005)
Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization.Developmental biology, 284 1
Gang Liang, Xiao Zhang, Lu Wang, Y. Sha, Jian Zhang, S. Miao, S. Zong, Lin Wang, S. Koide (2004)
Identification of differentially expressed genes of primary spermatocyte against round spermatid isolated from human testis using the laser capture microdissection techniqueCell Research, 14
K. Yoshinaga, K. Toshimori (2003)
Organization and modifications of sperm acrosomal molecules during spermatogenesis and epididymal maturationMicroscopy Research and Technique, 61
D. Mortimer, E. Curtis, R. Miller (1987)
Specific labelling by peanut agglutinin of the outer acrosomal membrane of the human spermatozoon.Journal of reproduction and fertility, 81 1
K. Kretz, D. Cripe, G. Carson, H. Fukushima, J. O'brien (1992)
Structure and sequence of the human alpha-L-fucosidase gene and pseudogene.Genomics, 12 2
W. White, K. Schray, G. Legler, J. Alhadeff (1987)
Further studies on the catalytic mechanism of human liver alpha-L-fucosidase.Biochimica et biophysica acta, 912 1
H. Lucas, S. Bercegeay, J. Pendu, M. Jean, S. Mirallié, P. Barrière (1994)
A fucose-containing epitope potentially involved in gamete interaction on the human zona pellucida.Human reproduction, 9 8
J. Ramalho‐Santos, G. Schatten, R. Moreno (2002)
Control of Membrane Fusion During Spermiogenesis and the Acrosome Reaction1, 67
Jean Kanitakis, C. Allombert, B. Doebelin, M. Deroo-Berger, S. Grande, S. Blanc, A. Claudy (2005)
Fucosidosis with angiokeratoma. Immunohistochemical & electronmicroscopic study of a new case and literature reviewJournal of Cutaneous Pathology, 32
Bounchanh Vannapraseuth (2005)
WHO Laboratory Manual for the Examination of Human Semen and Sperm-cervical Mucus Interaction 4th Edition [Book Review]The Australian Journal of Medical Science, 26
J. Alhadeff, S. Khunsook, K. Choowongkomon, T. Baney, V. Heredia, A. Tweedie, B. Bean (1999)
Characterization of human semen α-l-fucosidasesMolecular Human Reproduction, 5
J. Auer, L. Camoin, A. Courtot, F. Hotellier, M. Almeida (2004)
Evidence that P36, a human sperm acrosomal antigen involved in the fertilization process is triosephosphate isomeraseMolecular Reproduction and Development, 68
C. Allen, D. Green (1995)
Monoclonal antibodies which recognize equatorial segment epitopes presented de novo following the A23187-induced acrosome reaction of guinea pig sperm.Journal of cell science, 108 ( Pt 2)
E. Staudacher, F. Altmann, I. Wilson, L. März (1999)
Fucose in N-glycans: from plant to man.Biochimica et biophysica acta, 1473 1
S. Khunsook, J. Alhadeff, B. Bean (2002)
Purification and characterization of human seminal plasma alpha-L-fucosidase.Molecular human reproduction, 8 3
P. James, Conor Hennessy, T. Berge, Roy Jones (2004)
Compartmentalisation of the sperm plasma membrane: a FRAP, FLIP and SPFI analysis of putative diffusion barriers on the sperm headJournal of Cell Science, 117
Nirmala Jagadish, Ritu Rana, D. Mishra, M. Kumar, A. Suri (2005)
Sperm associated antigen 9 (SPAG9): a new member of c-Jun NH2 -terminal kinase (JNK) interacting protein exclusively expressed in testis.The Keio journal of medicine, 54 2
S. Khunsook, B. Bean, S. Mcgowan, J. Alhadeff (2003)
Purification and Characterization of Plasma Membrane-AssociatedHuman Sperm α-l-Fucosidase1, 68
J. Flechon, J. Degrouard, V. Kopečný, J. Pivko, A. Pavlok, J. Motlík
Reproductive Biology and Endocrinology Open Access the Extracellular Matrix of Porcine Mature Oocytes: Origin, Composition and Presumptive Roles
V. Ros, M. Munuce, D. Cohen, C. Marín-Briggiler, D. Busso, P. Visconti, P. Cuasnicú (2004)
Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability1, 70
D. Saxena, K. Toshimori (2004)
Molecular Modifications of MC31/CE9, a Sperm Surface Molecule, During Sperm Capacitation and the Acrosome Reaction in the Rat: Is MC31/CE9 Required for Fertilization?1, 70
D. Becker, J. Lowe (2003)
Fucose: biosynthesis and biological function in mammals.Glycobiology, 13 7
J. Intra, F. Cenni, M. Perotti (2006)
An α‐L‐fucosidase potentially involved in fertilization is present on Drosophila spermatozoa surfaceMolecular Reproduction and Development, 73
S. Suarez, D. Wolf, S. Meizel (1986)
Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluidGamete Research, 14
Stephen Johnson, J. Alhadeff (1991)
Mammalian a-l-fucosidasesComparative Biochemistry and Physiology B
W. White, K. Schray, G. Legler, J. Alhadeff (1987)
Further studies on the catalytic mechanism of human liver α-l-fucosidaseBiochimica et Biophysica Acta, 912
O. Berteau, J. Bielicki, A. Kilonda, Delphine Machy, D. Anson, L. Kenne (2004)
α-l-Fucosidases: Exoglycosidases with Unusual Transglycosylation PropertiesBiochemistry, 43
K. Kretz, D. Cripe, G. Carson, H. Fukushima, J. O'brien (1992)
Structure and sequence of the human α-l-fucosidase gene and pseudogeneGenomics, 12
O. Berteau, J. Bielicki, A. Kilonda, Delphine Machy, D. Anson, L. Kenne (2004)
Alpha-L-fucosidases: exoglycosidases with unusual transglycosylation properties.Biochemistry, 43 24
G. Andrews-Smith, J. Alhadeff (1982)
Radioimmunoassay determination of decreased amounts of alpha-L-fucosidase protein in fucosidosis.Biochimica et biophysica acta, 715 1
M. Matsumoto, J. Hirata, N. Hirohashi, M. Hoshi (2002)
Sperm-Egg Binding Mediated by Sperm α-l-Fucosidase in the Ascidian, Halocynthia roretzi, 19
M. Spinaci, S. Volpe, C. Bernardini, M. Ambrogi, C. Tamanini, E. Seren, G. Galeati (2005)
Immunolocalization of heat shock protein 70 (Hsp 70) in boar spermatozoa and its role during fertilizationMolecular Reproduction and Development, 72
B. Winchester, B. Winchester, C. Barker, S. Baines, G. Jacob, G. Jacob, S. Namgoong, G. Fleet (1990)
Inhibition of alpha-L-fucosidase by derivatives of deoxyfuconojirimycin and deoxymannojirimycin.The Biochemical journal, 265 1
B. Gadella, M. Lopes‐Cardozo, L. Golde, B. Colenbrander, Th.W.J. Gadella (1995)
Glycolipid migration from the apical to the equatorial subdomains of the sperm head plasma membrane precedes the acrosome reaction. Evidence for a primary capacitation event in boar spermatozoa.Journal of cell science, 108 ( Pt 3)
T. Hoodbhoy, J. Dean (2004)
Insights into the molecular basis of sperm-egg recognition in mammals.Reproduction, 127 4
M. Wolkowicz, J. Shetty, Anne Westbrook, K. Klotz, F. Jayes, A. Mandal, C. Flickinger, J. Herr (2003)
Equatorial Segment Protein Defines a Discrete Acrosomal Subcompartment Persisting Throughout Acrosomal Biogenesis1, 69
Two distinctive isoforms of the enzyme α‐L‐fucosidase are found within human semen in substantial amounts, suggesting specialized functions during reproduction. The membrane‐associated isozyme of human sperm cells was previously characterized biochemically, and here we report on its subcellular localization. Intact, detergent permeabilized, capacitated, and acrosome‐reacted sperm were investigated using antifucosidase immunofluorescence, binding of the fluorescent fucosylated glycoconjugate RITC‐BSA‐fucose (RBF), and enzyme activity in the presence and absence of selected inhibitors. Both immunolocalization and RBF binding show that fucosidase is broadly distributed over the membrane systems of human sperm, but is relatively enriched within the equatorial segment. Upon detergent treatment or induction of acrosome reaction (AR), a portion of enzyme activity is recoverable in the supernatant, presumably associated with released remnants of the outer acrosomal membrane. Surprisingly, cell‐bound enzyme activity increases sharply following permeabilization of intact sperm, representing cryptic fucosidase that is relatively stable and corresponds with strong fluorescence in the equatorial segment and other sperm membranes. These observations support the notion that the fucosidase has a role in the intimate species signature interactions between sperm and oocyte. Mol. Reprod. Dev. 74: 758–766, 2007. © 2006 Wiley‐Liss, Inc.
Molecular Reproduction & Development – Wiley
Published: Jun 1, 2007
Keywords: fertilization; gamete; equatorial segment; acrosome; fucose; DFJ
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