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Cell electrophoretic mobility and cationic flux

Cell electrophoretic mobility and cationic flux 10.1002/jcp.1040730105.abs The net negativity of the surfaces of Ehrlich ascites cells was reduced by treating them with either neuraminidase or ribonuclease. Neither enzyme treatment affected the Na+ or K+ content of the cells, before or after cooling at 4°C. Experiments with K42 revealed a reduction (9.5 to 17%) in unidirectional K+‐fluxes following incubation with neuraminidase, but no change after ribonuclease‐treatment. Our data suggest that surface anionic sites associated with RNA and sialic acid moieties are not of major quantitative importance in regulating either intracellular Na+ and K+ concentrations, or unidirectional transmembrane K+‐flux. Our results do not enable us to determine whether ion‐binding to anionic sites at the electro‐kinetic surface is not an essential prerequisite to transmembrane movement, or whether it is essential, but occurs through the 40% of cell surface net negativity which is unaffected by ribonuclease‐ and neuraminidase‐treatment. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Physiology Wiley

Cell electrophoretic mobility and cationic flux

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References (26)

Publisher
Wiley
Copyright
Copyright © 1969 Wiley‐Liss, Inc.
ISSN
0021-9541
eISSN
1097-4652
DOI
10.1002/jcp.1040730105
pmid
5765777
Publisher site
See Article on Publisher Site

Abstract

10.1002/jcp.1040730105.abs The net negativity of the surfaces of Ehrlich ascites cells was reduced by treating them with either neuraminidase or ribonuclease. Neither enzyme treatment affected the Na+ or K+ content of the cells, before or after cooling at 4°C. Experiments with K42 revealed a reduction (9.5 to 17%) in unidirectional K+‐fluxes following incubation with neuraminidase, but no change after ribonuclease‐treatment. Our data suggest that surface anionic sites associated with RNA and sialic acid moieties are not of major quantitative importance in regulating either intracellular Na+ and K+ concentrations, or unidirectional transmembrane K+‐flux. Our results do not enable us to determine whether ion‐binding to anionic sites at the electro‐kinetic surface is not an essential prerequisite to transmembrane movement, or whether it is essential, but occurs through the 40% of cell surface net negativity which is unaffected by ribonuclease‐ and neuraminidase‐treatment.

Journal

Journal of Cellular Physiology – Wiley

Published: Feb 1, 1969

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