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BhSGAMP‐1, a gene that encodes an antimicrobial peptide, is developmentally regulated by the direct action of 20‐OH ecdysone in the salivary gland of Bradysia hygida (Diptera, Sciaridae)

BhSGAMP‐1, a gene that encodes an antimicrobial peptide, is developmentally regulated by the... Recently we have shown that BhSGAMP‐1 is a developmentally regulated reiterated gene that encodes an antimicrobial peptide (AMP) and is expressed exclusively in the salivary glands, at the end of the larval stage. We show, for the first time, that a gene for an AMP is directly activated by 20‐OH ecdysone. This control probably involves the participation of short‐lived repressor(s). We also found that the promoter of BhSGAMP‐1 is not equipped with elements that respond to infection, provoked by the injection of microorganisms, in the salivary glands or in the fat body. We produced polyclonal antibodies against the synthetic peptide and found that the BhSGAMP‐1 peptide is secreted in the saliva. The BhSGAMP‐1 gene was also activated during the third larval molt. These facts confirm our hypothesis that this preventive system of defense was selected to produce an environment free of harmful microorganisms in the insect's immediate vicinity, during molts. genesis 47:847–857, 2009. © 2009 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genesis: the Journal of Genetics and Development Wiley

BhSGAMP‐1, a gene that encodes an antimicrobial peptide, is developmentally regulated by the direct action of 20‐OH ecdysone in the salivary gland of Bradysia hygida (Diptera, Sciaridae)

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References (56)

Publisher
Wiley
Copyright
Copyright © 2009 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1526-954X
eISSN
1526-968X
DOI
10.1002/dvg.20576
pmid
19882668
Publisher site
See Article on Publisher Site

Abstract

Recently we have shown that BhSGAMP‐1 is a developmentally regulated reiterated gene that encodes an antimicrobial peptide (AMP) and is expressed exclusively in the salivary glands, at the end of the larval stage. We show, for the first time, that a gene for an AMP is directly activated by 20‐OH ecdysone. This control probably involves the participation of short‐lived repressor(s). We also found that the promoter of BhSGAMP‐1 is not equipped with elements that respond to infection, provoked by the injection of microorganisms, in the salivary glands or in the fat body. We produced polyclonal antibodies against the synthetic peptide and found that the BhSGAMP‐1 peptide is secreted in the saliva. The BhSGAMP‐1 gene was also activated during the third larval molt. These facts confirm our hypothesis that this preventive system of defense was selected to produce an environment free of harmful microorganisms in the insect's immediate vicinity, during molts. genesis 47:847–857, 2009. © 2009 Wiley‐Liss, Inc.

Journal

Genesis: the Journal of Genetics and DevelopmentWiley

Published: Dec 1, 2009

Keywords: ; ; ; ; ;

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