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Automatic control for fed‐batch culture of single cell protein

Automatic control for fed‐batch culture of single cell protein 10.1002/bit.260230709.abs Candida utilis was cultivated in a 5‐liter jar fermentor using ethanol as sole carbon source. Control of ethanol in the cultivation broth was performed by using an ethanol vapor monitoring instrument and an oxygen electrode coupled with two control circuits. By setting upper and lower bounds according to the predetermined conditions, a signal from a gas monitoring sensor switched the lower or higher bound relay governing the actuating or switch‐off of the motor; this maintained a proper concentration of ethanol in the cultivation of ethanol in the system. The growth of cells was found to be satisfactory. Cell concentration reached 64 g/liter during a 20‐hr cultivation. As the results of comparative experiments, the control mode using the gas monitoring instrument was found to be superior to that using dissolved oxygen as a controlling signal, especially at high cell concentration. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

Automatic control for fed‐batch culture of single cell protein

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References (12)

Publisher
Wiley
Copyright
Copyright © 1981 John Wiley & Sons, Inc.
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.260230709
Publisher site
See Article on Publisher Site

Abstract

10.1002/bit.260230709.abs Candida utilis was cultivated in a 5‐liter jar fermentor using ethanol as sole carbon source. Control of ethanol in the cultivation broth was performed by using an ethanol vapor monitoring instrument and an oxygen electrode coupled with two control circuits. By setting upper and lower bounds according to the predetermined conditions, a signal from a gas monitoring sensor switched the lower or higher bound relay governing the actuating or switch‐off of the motor; this maintained a proper concentration of ethanol in the cultivation of ethanol in the system. The growth of cells was found to be satisfactory. Cell concentration reached 64 g/liter during a 20‐hr cultivation. As the results of comparative experiments, the control mode using the gas monitoring instrument was found to be superior to that using dissolved oxygen as a controlling signal, especially at high cell concentration.

Journal

Biotechnology and BioengineeringWiley

Published: Jul 1, 1981

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