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Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various... Nonporous and porous membranes from poly(L/DL‐lactide) 80/20% were treated with low‐temperature oxygen, ammonia, or sulphur dioxide–hydrogen plasmas and the late effects of plasma treatment on physicochemical characteristics of the membranes' surface were analyzed. The plasma treatment resulted in the permanent attachment of sulphur and nitrogen functionalities to the membrane's surface, and increased the surface concentration of oxygen, thereby increasing the surface wettability. To assess whether the plasma treatment affects the cellular response, primary rat osteoblasts were cultured on nontreated and plasma‐treated nonporous and microporous membranes, and attachment, growth, and activity of cells were investigated. It was found that attachment and growth of osteoblasts on all the plasma‐treated membranes were greater compared with nontreated controls. The treatment with ammonia plasma was most efficacious. The beneficial effects of plasma treatment on cells were most pronounced for microporous polylactide membranes irrespective of the plasma used. The results of the study suggest that the treatment of porous polylactide structures with plasma can be an effective means of enhancing their suitability for tissue engineering. Plasma exposure may also have an advantageous effect on bone healing when polylactide membranes are used to treat bone defects. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Materials Research Part A Wiley

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

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References (47)

Publisher
Wiley
Copyright
Copyright © 2006 Wiley Periodicals, Inc., A Wiley Company
ISSN
1549-3296
eISSN
1552-4965
DOI
10.1002/jbm.a.30462
pmid
16270339
Publisher site
See Article on Publisher Site

Abstract

Nonporous and porous membranes from poly(L/DL‐lactide) 80/20% were treated with low‐temperature oxygen, ammonia, or sulphur dioxide–hydrogen plasmas and the late effects of plasma treatment on physicochemical characteristics of the membranes' surface were analyzed. The plasma treatment resulted in the permanent attachment of sulphur and nitrogen functionalities to the membrane's surface, and increased the surface concentration of oxygen, thereby increasing the surface wettability. To assess whether the plasma treatment affects the cellular response, primary rat osteoblasts were cultured on nontreated and plasma‐treated nonporous and microporous membranes, and attachment, growth, and activity of cells were investigated. It was found that attachment and growth of osteoblasts on all the plasma‐treated membranes were greater compared with nontreated controls. The treatment with ammonia plasma was most efficacious. The beneficial effects of plasma treatment on cells were most pronounced for microporous polylactide membranes irrespective of the plasma used. The results of the study suggest that the treatment of porous polylactide structures with plasma can be an effective means of enhancing their suitability for tissue engineering. Plasma exposure may also have an advantageous effect on bone healing when polylactide membranes are used to treat bone defects. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

Journal

Journal of Biomedical Materials Research Part AWiley

Published: Feb 1, 2006

Keywords: polylactide; membranes; plasma treatment; osteoblasts; cell culture

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