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Philippe Tartaix, Marie Doulaverakis, A. George, L. Fisher, W. Butler, C. Qin, E. Salih, M. Tan, Y. Fujimoto, L. Spevak, A. Boskey (2004)
In Vitro Effects of Dentin Matrix Protein-1 on Hydroxyapatite Formation Provide Insights into in Vivo Functions*Journal of Biological Chemistry, 279
J. Westermarck, E. Ilvonen, V. Kähäri (1995)
The protein phosphatase inhibitor okadaic acid suppresses type I collagen gene expression in cultured fibroblasts at the transcriptional level.The Biochemical journal, 308 ( Pt 3)
Y. Kato, A. Boskey, L. Spevak, Mark Dallas, M. Hori, L. Bonewald (2001)
Establishment of an Osteoid Preosteocyte‐like Cell MLO‐A5 That Spontaneously Mineralizes in CultureJournal of Bone and Mineral Research, 16
Hiran Perinpanayagam, R. Zaharias, C. Stanford, R. Brand, J. Keller, G. Schneider (2001)
Early cell adhesion events differ between osteoporotic and non‐osteoporotic osteoblastsJournal of Orthopaedic Research, 19
Yasuhide Furuta, Dusko Ilic, Satoshi Kanazawa, Naoki Takeda, Tadashi Yamamoto, Shinichi Aizawa (1995)
Mesodermal defect in late phase of gastrulation by a targeted mutation of focal adhesion kinase, FAK.Oncogene, 11 10
J. Gutierrez, H. Perr (1999)
Mechanical stretch modulates TGF- b 1 and a 1(I) collagen expression in fetal human intestinal smooth muscle cells
C. Toma, M. Pittenger, K. Cahill, B. Byrne, P. Kessler (2002)
Human Mesenchymal Stem Cells Differentiate to a Cardiomyocyte Phenotype in the Adult Murine HeartCirculation: Journal of the American Heart Association, 105
A. Utani, Y. Momota, H. Endo, Y. Kasuya, K. Beck, N. Suzuki, M. Nomizu, H. Shinkai (2003)
Laminin α3 LG4 Module Induces Matrix Metalloproteinase-1 through Mitogen-activated Protein Kinase Signaling*Journal of Biological Chemistry, 278
P. Wieczorek, J. Zawierta, R. Rzeuski (2000)
[Mutations of genes coding collagen type I--biochemical and clinical effects].Postepy higieny i medycyny doswiadczalnej, 54 1
Hector Rios, Ling Ye, V. Dusevich, D. Eick, Lynda Bonewald, Jian Feng (2005)
DMP1 is essential for osteocyte formation and function.Journal of musculoskeletal & neuronal interactions, 5 4
M. Pittenger, A. Mackay, S. Beck, R. Jaiswal, Robin Douglas, J. Mosca, M. Moorman, D. Simonetti, S. Craig, D. Marshak (1999)
Multilineage potential of adult human mesenchymal stem cells.Science, 284 5411
G. Plopper, S. Domanico, V. Cirulli, W. Kiosses, V. Quaranta (1998)
Migration of breast epithelial cells on Laminin-5: differential role of integrins in normal and transformed cell typesBreast Cancer Research and Treatment, 51
JA Jadlowiec, X Zhang, J Li, PG Campbell, C Sfeir (2005)
ECM‐mediated signaling by dentin phosphophoryn involves activation of the Smad pathway independent of BMP, 281
L. Gowen, D. Petersen, A. Mansolf, H. Qi, J. Stock, G. Tkalcevic, H. Simmons, D. Crawford, K. Chidsey-Frink, H. Ke, J. Mcneish, T. Brown (2003)
Targeted Disruption of the Osteoblast/Osteocyte Factor 45 Gene (OF45) Results in Increased Bone Formation and Bone Mass*The Journal of Biological Chemistry, 278
C. Qin, J. Brunn, Elizabeth Cadena, A. Ridall, H. Tsujigiwa, H. Nagatsuka, N. Nagai, W. Butler (2002)
The Expression of Dentin Sialophosphoprotein Gene in BoneJournal of Dental Research, 81
N. Datta, Quynh Pham, U. Sharma, V. Sikavitsas, J. Jansen, A. Mikos (2006)
In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation.Proceedings of the National Academy of Sciences of the United States of America, 103 8
(2004)
In vitro effects of dentinmatrix protein
A. Utani, Y. Momota, H. Endo, Y. Kasuya, K. Beck, N. Suzuki, M. Nomizu, H. Shinkai (2003)
Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling.The Journal of biological chemistry, 278 36
N. Jaiswal, S. Haynesworth, A. Caplan, S. Bruder (1997)
Osteogenic differentiation of purified, culture‐expanded human mesenchymal stem cells in vitroJournal of Cellular Biochemistry, 64
A. Mackay, S. Beck, J. Murphy, F. Barry, C. Chichester, M. Pittenger (1998)
Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow.Tissue engineering, 4 4
M. Owen, A. Friedenstein (1988)
Stromal stem cells: marrow-derived osteogenic precursors.Ciba Foundation symposium, 136
P. Manduca, S. Marchisio, S. Astigiano, S. Zanotti, F. Galmozzi, C. Palermo, D. Palmieri (2005)
FMS * Calciumfluor specifically increases mRNA levels and induces signaling via MAPK 42,44 and not FAK in differentiating rat osteoblastsCell Biology International, 29
C. Clemente, M. Corat, S. Saad, K. Franchini (2005)
Differentiation of C2C12 myoblasts is critically regulated by FAK signaling.American journal of physiology. Regulatory, integrative and comparative physiology, 289 3
V. Meyers, M. Zayzafoon, S. Gonda, W. Gathings, J. McDonald (2013)
Modeled microgravity disrupts collagen I/integrin signaling during osteoblastic differentiation of human mesenchymal stem cellsJournal of Cellular Biochemistry, 93
J. Jadlowiec, Xiaoyuan Zhang, Jinhua Li, P. Campbell, C. Sfeir (2006)
Extracellular Matrix-mediated Signaling by Dentin Phosphophoryn Involves Activation of the Smad Pathway Independent of Bone Morphogenetic Protein*Journal of Biological Chemistry, 281
Xu Peng, M. Kraus, Huijun Wei, Tang-Long Shen, R. Pariaut, A. Alcaraz, Guangju Ji, Lihong Cheng, Qinglin Yang, M. Kotlikoff, Ju Chen, K. Chien, Hua Gu, J. Guan (2005)
Inactivation of focal adhesion kinase in cardiomyocytes promotes eccentric cardiac hypertrophy and fibrosis in mice.The Journal of clinical investigation, 116 1
G. Mclean, N. Komiyama, B. Serrels, H. Asano, L. Reynolds, F. Conti, K. Hodivala-Dilke, D. Metzger, P. Chambon, S. Grant, M. Frame (2004)
Specific deletion of focal adhesion kinase suppresses tumor formation and blocks malignant progression.Genes & development, 18 24
Robert Klees, Roman Salasznyk, K. Kingsley, William Williams, A. Boskey, G. Plopper (2004)
Laminin-5 induces osteogenic gene expression in human mesenchymal stem cells through an ERK-dependent pathway.Molecular biology of the cell, 16 2
Frank Kotch, R. Raines (2006)
Self-assembly of synthetic collagen triple helices.Proceedings of the National Academy of Sciences of the United States of America, 103 9
Junko Hashimoto, Takashi Ogawa, Y. Tsubota, K. Miyazaki (2005)
Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5.Experimental cell research, 310 2
R. Jaiswal, N. Jaiswal, S. Bruder, G. Mbalaviele, D. Marshak, M. Pittenger (2000)
Adult Human Mesenchymal Stem Cell Differentiation to the Osteogenic or Adipogenic Lineage Is Regulated by Mitogen-activated Protein Kinase*The Journal of Biological Chemistry, 275
K. Nakashima, Xin Zhou, G. Kunkel, Zhaoping Zhang, J. Deng, R. Behringer, B. Crombrugghe (2002)
The Novel Zinc Finger-Containing Transcription Factor Osterix Is Required for Osteoblast Differentiation and Bone FormationCell, 108
K. Narayanan, R. Srinivas, A. Ramachandran, J. Hao, B. Quinn, A. George (2001)
Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1Proceedings of the National Academy of Sciences of the United States of America, 98
Rena D'Souza, A. Cavender, G. Sunavala, J. Alvarez, Toshio Ohshima, Ashok Kulkarni, Mary Macdougall (1997)
Gene Expression Patterns of Murine Dentin Matrix Protein 1 (Dmp1) and Dentin Sialophosphoprotein (DSPP) Suggest Distinct Developmental Functions In VivoJournal of Bone and Mineral Research, 12
G. Xiao, Di Jiang, P. Thomas, M. Benson, K. Guan, G. Karsenty, R. Franceschi (2000)
MAPK Pathways Activate and Phosphorylate the Osteoblast-specific Transcription Factor, Cbfa1*The Journal of Biological Chemistry, 275
Jian Feng, Haiyang Huang, Haiyang Huang, Yongbo Lu, L. Ye, Y. Xie, T. Tsutsui, T. Kunieda, T. Kunieda, T. Castranio, Greg Scott, L. Bonewald, Y. Mishina (2003)
The Dentin Matrix Protein 1 (Dmp1) is Specifically Expressed in Mineralized, but not Soft, Tissues during DevelopmentJournal of Dental Research, 82
Y. Takeuchi, M. Suzawa, T. Kikuchi, E. Nishida, T. Fujita, T. Matsumoto (1997)
Differentiation and transforming growth factor-beta receptor down-regulation by collagen-alpha2beta1 integrin interaction is mediated by focal adhesion kinase and its downstream signals in murine osteoblastic cells.The Journal of biological chemistry, 272 46
Roman Salasznyk, William Williams, A. Boskey, Anna Batorsky, G. Plopper (2004)
Adhesion to Vitronectin and Collagen I Promotes Osteogenic Differentiation of Human Mesenchymal Stem CellsJournal of Biomedicine and Biotechnology, 2004
Y. Takeuchi, M. Suzawa, T. Kikuchi, E. Nishida, T. Fujita, Toshio Matsumoto (1997)
Differentiation and Transforming Growth Factor-β Receptor Down-regulation by Collagen-α2β1 Integrin Interaction Is Mediated by Focal Adhesion Kinase and Its Downstream Signals in Murine Osteoblastic Cells*The Journal of Biological Chemistry, 272
Tang-Long Shen, Ann Park, A. Alcaraz, Xu Peng, I. Jang, P. Koni, R. Flavell, Hua Gu, J. Guan (2005)
Conditional knockout of focal adhesion kinase in endothelial cells reveals its role in angiogenesis and vascular development in late embryogenesisThe Journal of Cell Biology, 169
M. Macdougall, D. Simmons, X. Luan, J. Nydegger, Jian Feng, T. Gu (1997)
Dentin Phosphoprotein and Dentin Sialoprotein Are Cleavage Products Expressed from a Single Transcript Coded by a Gene on Human Chromosome 4The Journal of Biological Chemistry, 272
D. Hakuno, Tomosaburo Takahashi, J. Lammerding, Richard Lee (2005)
Focal Adhesion Kinase Signaling Regulates Cardiogenesis of Embryonic Stem Cells*Journal of Biological Chemistry, 280
D. Gullberg, E. Lundgren-Åkerlund (2002)
Collagen-binding I domain integrins--what do they do?Progress in histochemistry and cytochemistry, 37 1
M MacDougall, D Simmons, X Luan, J Nydegger, J Feng, TT Gu (1997)
Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4. Dentin phosphoprotein DNA sequence determination, 272
S. Mitra, Daniel Hanson, D. Schlaepfer (2005)
Focal adhesion kinase: in command and control of cell motilityNature Reviews Molecular Cell Biology, 6
Yeon-Ju Kim, Hyun‐Nam Kim, E. Park, Byungheon Lee, H. Ryoo, Shin-Yoon Kim, In‐San Kim, J. Stein, J. Lian, G. Stein, A. Wijnen, Je-Yong Choi (2006)
The bone-related Zn finger transcription factor Osterix promotes proliferation of mesenchymal cells.Gene, 366 1
M. Kjaer (2004)
Role of extracellular matrix in adaptation of tendon and skeletal muscle to mechanical loading.Physiological reviews, 84 2
Roman Salasznyk, Robert Klees, Mariah Hughlock, G. Plopper (2004)
ERK Signaling Pathways Regulate the Osteogenic Differentiation of Human Mesenchymal Stem Cells on Collagen I and VitronectinCell Communication & Adhesion, 11
George Plopper, J. Falk-Marzillier, Scott Glaser, M. Fitchmun, Gianluigi Giannelli, Tracy Romano, Jonathan Jones, Vito Quaranta (1996)
Changes in expression of monoclonal antibody epitopes on laminin-5r induced by cell contact.Journal of cell science, 109 ( Pt 7)
G. Tai, I. Christodoulou, A. Bishop, J. Polak (2005)
Use of green fluorescent fusion protein to track activation of the transcription factor osterix during early osteoblast differentiation.Biochemical and biophysical research communications, 333 4
J. Jadlowiec, H. Koch, Xiaoyuan Zhang, P. Campbell, Merriam Seyedain, C. Sfeir (2004)
Phosphophoryn Regulates the Gene Expression and Differentiation of NIH3T3, MC3T3-E1, and Human Mesenchymal Stem Cells via the Integrin/MAPK Signaling Pathway*Journal of Biological Chemistry, 279
T. Hayashibara, T. Hiraga, B. Yi, M. Nomizu, Y. Kumagai, R. Nishimura, T. Yoneda (2003)
A Synthetic Peptide Fragment of Human MEPE Stimulates New Bone Formation In Vitro and In VivoJournal of Bone and Mineral Research, 19
S. Essayem, Branka Kovacic-Milivojevic, Clark Baumbusch, Susan McDonagh, Gregory Dolganov, Kyle Howerton, N. Larocque, T. Mauro, Angel Ramirez, Daniel Ramos, Susan Fisher, J. Jorcano, Hilary Beggs, Louis Reichardt, D. Ilić (2006)
Hair cycle and wound healing in mice with a keratinocyte-restricted deletion of FAKOncogene, 25
Human mesenchymal stem cell (hMSC) differentiation into osteoblasts and the signaling events involved are poorly understood. We recently established that contact with specific extracellular matrix (ECM) proteins, in particular laminin‐5, is sufficient to induce an osteogenic phenotype in hMSC through an extracellular signal‐related kinase (ERK)‐dependent pathway. Activation of ERK 1/2 by laminin‐5 induces phosphorylation of the runx2/cbfa‐1 transcription factor that controls osteogenic gene expression. We hypothesized that focal adhesion kinase (FAK) mediated signaling pathways supply a link between cell surface integrin‐ECM binding and activation of ERK 1/2, and that laminin‐5 promotes its osteogenic effects through this pathway. To test this hypothesis, we plated hMSC on a laminin‐5 matrix in the presence or absence of FAK‐specific small inhibitory RNAs (siRNA), and assayed for phosphorylation of runx2/cbfa‐1 as well as expression of established osteogenic differentiation markers (bone sialoprotein, osteocalcin, alkaline phosphatase, calcium deposition, and mineral:matrix ratio). We found that siRNA treatment reduced total endogenous FAK protein by ∼40%, and reduced FAK phosphorylation on Y397 by ∼33% in cells plated on laminin‐5 for 30 min. SiRNA treated cells exhibited a decrease in ERK 1/2 phosphorylation after 1 h, and reduced serine/threonine phosphorylation of Runx2/Cbfa‐1 after 8 days. Finally, FAK inhibition blocked osteogenic differentiation of hMSC, as assessed by lowered expression of osteogenic genes (RT‐PCR), decreased alkaline phosphatase activity, greatly reduced calcium deposition, and a lower mineral:matrix ratio after 28 days in culture. These results establish FAK as an important mediator of laminin‐5‐induced osteogenic differentiation of hMSC. J. Cell. Biochem. 100: 499–514, 2007. © 2006 Wiley‐Liss, Inc.
Journal of Cellular Biochemistry – Wiley
Published: Jan 1, 2007
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