Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Acrosome reaction of boar spermatozoa in homologous in vitro fertilization

Acrosome reaction of boar spermatozoa in homologous in vitro fertilization 10.1002/mrd.1080360112.abs Ejaculated spermatozoa from four different boars were used to evaluate the acrosome reaction during in vitro fertilization with homologous ovulated oocytes. The acrosome reaction was assessed according to a peroxidase‐labeling peanut agglutinin method and a triple‐stain technique. An increase in the proportion of living sperm with reacted acrosomes was observed after preincubation and 2 hr of coincubation (P < 0.05). The percentage of true acrosome‐reacted sperm remained reasonably constant throughout coincubation. In vitro penetration rates of oocytes varied among boars, but no relationship was found between fertilization rates of oocytes and maximum percentages of acrosome reacted living sperm. © 1993 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Reproduction & Development Wiley

Acrosome reaction of boar spermatozoa in homologous in vitro fertilization

Download PDF
5 pages

Loading next page...
 
/lp/wiley/acrosome-reaction-of-boar-spermatozoa-in-homologous-in-vitro-WCfHNRu6dK

References (21)

Publisher
Wiley
Copyright
Copyright © 1993 Wiley‐Liss, Inc.
ISSN
1040-452X
eISSN
1098-2795
DOI
10.1002/mrd.1080360112
pmid
7691101
Publisher site
See Article on Publisher Site

Abstract

10.1002/mrd.1080360112.abs Ejaculated spermatozoa from four different boars were used to evaluate the acrosome reaction during in vitro fertilization with homologous ovulated oocytes. The acrosome reaction was assessed according to a peroxidase‐labeling peanut agglutinin method and a triple‐stain technique. An increase in the proportion of living sperm with reacted acrosomes was observed after preincubation and 2 hr of coincubation (P < 0.05). The percentage of true acrosome‐reacted sperm remained reasonably constant throughout coincubation. In vitro penetration rates of oocytes varied among boars, but no relationship was found between fertilization rates of oocytes and maximum percentages of acrosome reacted living sperm. © 1993 Wiley‐Liss, Inc.

Journal

Molecular Reproduction & Development – Wiley

Published: Sep 1, 1993

There are no references for this article.