Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Dunnen, G. Ommen (1999)
The protein truncation test: A reviewHuman Mutation, 14
A. Rowan, W. Bodmer (1997)
Introduction of a myc reporter tag to improve the quality of mutation detection using the protein truncation test.Human mutation, 9 2
(2001)
A new method for detecting DNA mutations
R. Luijt, P. Khan, H. Vasen, Claus Leeuwen, C. Tops, P. Roest, J. Dunnen, R. Fodde (1994)
Rapid detection of translation-terminating mutations at the adenomatous polyposis coli (APC) gene by direct protein truncation test.Genomics, 20 1
(2001)
Fluoretect Green Lys in vitro translation labelling system
Rowan (1997)
Introduction of a myc reporter tag to improve the quality of mutation detection using the protein truncation testHum Mutat, 9
S. Powell, G. Petersen, A. Krush, S. Booker, J. Jen, F. Giardiello, S. Hamilton, B. Vogelstein, K. Kinzler (1993)
Molecular diagnosis of familial adenomatous polyposis.The New England journal of medicine, 329 27
D. Sidransky (1997)
Nucleic acid-based methods for the detection of cancer.Science, 278 5340
R. Deuter, O. Müller (1998)
Detection of APC mutations in stool DNA of patients with colorectal cancer by HD‐PCRHuman Mutation, 11
Den Dunnen (1999)
The protein truncation test: a reviewHum Mutat, 14
ヴォーゲルスタイン,バート, キンズラー,ケネス・ダブリュー (1994)
Molecular diagnosis of familial adenomatous polyposis
P. Roest, R. Roberts, S. Sugino, G. Ommen, J. Dunnen (1993)
Protein truncation test (PTT) for rapid detection of translation-terminating mutations.Human molecular genetics, 2 10
A new method for mutation detection is described, which is a technical advancement of the protein truncation test. The new technique is non‐radioactive and highly sensitive for detection of virtually all sequence mutations, which lead to a stop signal or to the shift of the translation frame. The method includes four steps: 1) capture of the interesting sequence copies out of the sample by binding to an immobilized complementary sequence, 2) PCR amplification of the gene fragment to be analyzed with primers coding both for amino‐ and carboxy‐terminal tags, 3) in vitro transcription and translation, and 4) analysis of the translation products by Western blot. As an evaluation of the new method, we detected mutated gene copies at a dilution of 1 to 40 compared to the non‐mutated gene. Using the method, we were able to detect a mutation in the adenomatous polyposis coli tumor suppressor gene (APC) in a stool sample of a colorectal cancer patient. This mutation could not be detected by direct sequencing of the amplified APC gene fragment. Hum Mutat 19:165–172, 2002. © 2002 Wiley‐Liss, Inc.
Human Mutation – Wiley
Published: Feb 1, 2002
Keywords: adenomatous polyposis coli; APC; hybrid capture; in vitro synthesis protein assay; IVSP; mutation detection; protein truncation test; PTT; tumor suppressor
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.