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ROBERT NUSSBAUM FROMM, Division of Gastroenterology Nutrition, Department of Medicine, George Washington University Medical Center, Washington, District of Columbia 20037 Bouscarel, Bernard, Hans Fromm, Robert Nussbaum. Ursodeoxycholate intracellular Ca2+ phosphorylase a in isolated hepatocytes. Am. J. Physiol. 264(Gastrointest. Liver Physiol. 27): G&WG251,1993.-In isolated hamster hepatocytes, ursodeoxycholic acid () mobilized intracellular free calcium ([ Caâ+]J activated phosphorylase a with a half-maximally effective concentration of 188 9 PM, respectively. Addition of ethylene glycol-bis(P-aminoethyl ether)-N,N,Nâ,Nâ-tetraacetic acid (EGTA) did not affect the maximum [Ca2+]i mobilized by ; however, [Caââ]; returned to basal levels in 4-5 min compared with X0 min in the absence of EGTA. Both vasopressin activated phosphorylase a to the same extent in the presence absence of extracellular Ca2+, the effect of both agents was abolished when the cells were depleted in Ca2+. Vasopressin (100 nM) did not further mobilize [Ca2+]i or activate phosphorylase a when combined with 500 PM . However, unlike vasopressin, did not stimulate inositol 1,4,5-trisphosphate (IP,) formation. In contrast to taurine-conjugated (T), concentrations ~500 ,uM of glycine-conjugated (G) did not affect either [Ca2+]i or phosphorylase a . Lithocholic acid taurolithocholic acid (TLCA) displayed the highest affinity for Ca 2+. In addition, TLCA, chenodeoxycholic acid, NaF stimulated
AJP - Gastrointestinal and Liver Physiology – The American Physiological Society
Published: Feb 1, 1993
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