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Ursodeoxycholate mobilizes intracellular Ca2+ and activates phosphorylase a in isolated hepatocytes

Ursodeoxycholate mobilizes intracellular Ca2+ and activates phosphorylase a in isolated hepatocytes ROBERT NUSSBAUM FROMM, Division of Gastroenterology Nutrition, Department of Medicine, George Washington University Medical Center, Washington, District of Columbia 20037 Bouscarel, Bernard, Hans Fromm, Robert Nussbaum. Ursodeoxycholate intracellular Ca2+ phosphorylase a in isolated hepatocytes. Am. J. Physiol. 264(Gastrointest. Liver Physiol. 27): G&WG251,1993.-In isolated hamster hepatocytes, ursodeoxycholic acid () mobilized intracellular free calcium ([ Ca’+]J activated phosphorylase a with a half-maximally effective concentration of 188 9 PM, respectively. Addition of ethylene glycol-bis(P-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) did not affect the maximum [Ca2+]i mobilized by ; however, [Ca”‘]; returned to basal levels in 4-5 min compared with X0 min in the absence of EGTA. Both vasopressin activated phosphorylase a to the same extent in the presence absence of extracellular Ca2+, the effect of both agents was abolished when the cells were depleted in Ca2+. Vasopressin (100 nM) did not further mobilize [Ca2+]i or activate phosphorylase a when combined with 500 PM . However, unlike vasopressin, did not stimulate inositol 1,4,5-trisphosphate (IP,) formation. In contrast to taurine-conjugated (T), concentrations ~500 ,uM of glycine-conjugated (G) did not affect either [Ca2+]i or phosphorylase a . Lithocholic acid taurolithocholic acid (TLCA) displayed the highest affinity for Ca 2+. In addition, TLCA, chenodeoxycholic acid, NaF stimulated http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Gastrointestinal and Liver Physiology The American Physiological Society

Ursodeoxycholate mobilizes intracellular Ca2+ and activates phosphorylase a in isolated hepatocytes

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Publisher
The American Physiological Society
Copyright
Copyright © 1993 the American Physiological Society
ISSN
0193-1857
eISSN
1522-1547
Publisher site
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Abstract

ROBERT NUSSBAUM FROMM, Division of Gastroenterology Nutrition, Department of Medicine, George Washington University Medical Center, Washington, District of Columbia 20037 Bouscarel, Bernard, Hans Fromm, Robert Nussbaum. Ursodeoxycholate intracellular Ca2+ phosphorylase a in isolated hepatocytes. Am. J. Physiol. 264(Gastrointest. Liver Physiol. 27): G&WG251,1993.-In isolated hamster hepatocytes, ursodeoxycholic acid () mobilized intracellular free calcium ([ Ca’+]J activated phosphorylase a with a half-maximally effective concentration of 188 9 PM, respectively. Addition of ethylene glycol-bis(P-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) did not affect the maximum [Ca2+]i mobilized by ; however, [Ca”‘]; returned to basal levels in 4-5 min compared with X0 min in the absence of EGTA. Both vasopressin activated phosphorylase a to the same extent in the presence absence of extracellular Ca2+, the effect of both agents was abolished when the cells were depleted in Ca2+. Vasopressin (100 nM) did not further mobilize [Ca2+]i or activate phosphorylase a when combined with 500 PM . However, unlike vasopressin, did not stimulate inositol 1,4,5-trisphosphate (IP,) formation. In contrast to taurine-conjugated (T), concentrations ~500 ,uM of glycine-conjugated (G) did not affect either [Ca2+]i or phosphorylase a . Lithocholic acid taurolithocholic acid (TLCA) displayed the highest affinity for Ca 2+. In addition, TLCA, chenodeoxycholic acid, NaF stimulated

Journal

AJP - Gastrointestinal and Liver PhysiologyThe American Physiological Society

Published: Feb 1, 1993

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