Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Trafficking of apical proteins into clathrin-coated vesicles isolated from rat renal cortex

Trafficking of apical proteins into clathrin-coated vesicles isolated from rat renal cortex METHODS AND MATERIALS (CCV) are identified by the characteristic polyhedral clathrin baskets that cover them (3, 4, 17, 18). Originally identified in oocytes (4, ls>, CCV have been isolated from numerous cell lines and differentiated tissues (17, 26). Although CCV are reported in proximal tubular cells in electron microscopy studies (3>, they have not previously been isolated from the cortex. ’ The characteristics of CCV isolated from non tissues give important clues on isolation and identification of CCV. First, compared with most other intracellular organelles, CCV have an extremely light buoyant density (2, 4, 29). CCV can be heavily enriched in sucrose and heavy water gradients (2,4, 29). Second, in mammalian tissues such as the human placenta, CCV are the only intracellular structure known to lack mannose sugars (2). This can be exploited to utilize negative selection by lectin binding during purification (2, 4). Experience with CCV isolation in other tissues, F554 0363-6127/ Copyright Animals. Male Sprague-Dawley rats (ZOO-250 g) were purchased from Sasco, Omaha, NE. On the day of the experiment, rats were anesthetized with pentobarbital sodium. Through a midline incision, a tracheostomy was performed, and a catheter was placed in the left internal jugular vein. After http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Renal Physiology The American Physiological Society

Trafficking of apical proteins into clathrin-coated vesicles isolated from rat renal cortex

T. G. Hammond and P. J. Verroust
AJP - Renal Physiology , Volume 266: F554 – Apr 1, 1994
Download PDF
9 pages

Loading next page...
 
/lp/the-american-physiological-society/trafficking-of-apical-proteins-into-clathrin-coated-vesicles-isolated-KNatKOtgyF

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
The American Physiological Society
Copyright
Copyright © 1994 the American Physiological Society
ISSN
0363-6127
eISSN
1522-1466
Publisher site
See Article on Publisher Site

Abstract

METHODS AND MATERIALS (CCV) are identified by the characteristic polyhedral clathrin baskets that cover them (3, 4, 17, 18). Originally identified in oocytes (4, ls>, CCV have been isolated from numerous cell lines and differentiated tissues (17, 26). Although CCV are reported in proximal tubular cells in electron microscopy studies (3>, they have not previously been isolated from the cortex. ’ The characteristics of CCV isolated from non tissues give important clues on isolation and identification of CCV. First, compared with most other intracellular organelles, CCV have an extremely light buoyant density (2, 4, 29). CCV can be heavily enriched in sucrose and heavy water gradients (2,4, 29). Second, in mammalian tissues such as the human placenta, CCV are the only intracellular structure known to lack mannose sugars (2). This can be exploited to utilize negative selection by lectin binding during purification (2, 4). Experience with CCV isolation in other tissues, F554 0363-6127/ Copyright Animals. Male Sprague-Dawley rats (ZOO-250 g) were purchased from Sasco, Omaha, NE. On the day of the experiment, rats were anesthetized with pentobarbital sodium. Through a midline incision, a tracheostomy was performed, and a catheter was placed in the left internal jugular vein. After

Journal

AJP - Renal Physiology – The American Physiological Society

Published: Apr 1, 1994

There are no references for this article.