Abstract In the study presented, the hypothesis that ligands of the insulin-like growth factor (IGF) family, as well as their binding proteins (BPs), are temporally regulated and are altered by elevated progesterone (P4) and/or pregnancy was tested. Heifers detected in standing estrus following synchronization ( n = 210, day 0 ), were artificially inseminated ( n = 140) or left as noninseminated cyclic controls ( n = 70). On day 3 , half of each group were randomly assigned to receive a P4-releasing intravaginal device resulting in four treatment groups, pregnant and cyclic heifers with high and normal P4 concentrations on either day 5, 7, 13 , or 16 of the estrous cycle/early pregnancy. Quantitative real-time PCR and immunohistochemistry were performed on endometrial homogenate and uterine cross sections to measure transcript abundance and protein localization respectively ( n = 5 per treatment per time point). No effect of day, treatment, or their interactions was observed for IGF2 , IGFBP4 , and - 5 ( P > 0.05). IGF1 and IGFBP6 expression decreased, while IGF1R and IGFBP2 expression increased ( P < 0.05) as the days of the cycle or pregnancy progressed. The expression of IGFBP2 was increased by elevated P4; in addition, localization of both IGF2 and IGFBP2 protein in the luminal and superficial glandular epithelium displayed a P4 × day interaction. In conclusion it is proposed that decreased IGF1 and IGFBP6 expression with a coordinate increase in IGF1R and IGFBP2 as the estrous cycle/early pregnancy progresses, along with other factors, are required to establish a uterine environment that promotes the growth and development of the conceptus prior to implantation. In addition, the increased protein abundance of both IGF2 and IGFBP2 observed in heifers supplemented with P4 contributes to the enhanced conceptus elongation observed in this model. insulin-like growth factor 1 receptor insulin-like growth factor 2 insulin-like growth factor binding protein 2 insulin-like growth factor binding protein 6 bovine uterine estrus cycle Footnotes Copyright © 2012 the American Physiological Society « Previous | Next Article » Table of Contents This Article Published online before print November 2011 , doi: 10.​1152/​physiolgenomics.​00106.​2011 Physiol. Genomics January 2012 vol. 44 no. 2 130-140 » Abstract Free Full Text Free to you Full Text (PDF) Free to you Supplemental Table All Versions of this Article: physiolgenomics.00106.2011v1 44/2/130 most recent Classifications Research Article Services Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Citing Articles Load citing article information Citing articles via Web of Science Google Scholar Articles by McCarthy, S. D. Articles by Forde, N. PubMed PubMed citation Articles by McCarthy, S. D. Articles by Forde, N. Related Content Load related web page information Current Issue January 2012, 44 (2) Alert me to new issues of Physiol. Genomics About the Journal Information for Authors Submit a Manuscript Ethical Policies AuthorChoice PubMed Central Policy Reprints and Permissions Advertising Press Copyright © 2012 the American Physiological Society Print ISSN: 1094-8341 Online ISSN: 1531-2267 var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-2924550-1"); pageTracker._trackPageview();

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