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Substrate cycling between pyruvate and oxaloacetate in awake normal and 3,3'-5-triiodo-L-thyronine-treated rats

Substrate cycling between pyruvate and oxaloacetate in awake normal and... Petersen, Kitt Falk, Gary W. Cline, James B. Blair, and Gerald I. Shulman. between pyruvate and oxaloacetate in awake normal and 3,3’-&triiodo-Lthyronine-treated rats. Am. J. Physiol. 267 (Endocrinol. Metab. 30): E273-E277, 1994.- between pyruvate and oxaloacetate was assessed in awake 24-h fasted normal and triiodothyronine (T&treated rats. After a 20- or 60-min infusion of 13-13C]alanine (99% enriched, 12 mg/min) the 13C enrichments of liver glucose and alanine carbons were analyzed by 1.3Cand lH nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. from phosphoenolpyruvate to pyruvate [via pyruvate kinase (PK)] and from oxaloacetate to pyruvate [via malic enzyme (ME)] relative to the pyruvate carboxylase (PC) flux [i.e., (PK + ME)/PC] was assessed by the ratio of the 13C enrichment of C-2 alanine relative to that in C-5 glucose. In the normal rats (PK + ME)/PC was 0.26 t 0.07 (n = 7, t = 20 min) and 0.37 * 0.08 (n = 4, t = 60 min). In the T3-treated rats the (PK + ME)/PC increased four- to fivefold to 1.03 t 0.19 (n = 8, t = 20 min) and to 1.83 2 0.19 (n = 3, t = 60 min) (P < 0.05 vs. normal rats). The http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Endocrinology and Metabolism The American Physiological Society

Substrate cycling between pyruvate and oxaloacetate in awake normal and 3,3'-5-triiodo-L-thyronine-treated rats

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Publisher
The American Physiological Society
Copyright
Copyright © 1994 the American Physiological Society
ISSN
0193-1849
eISSN
1522-1555
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Abstract

Petersen, Kitt Falk, Gary W. Cline, James B. Blair, and Gerald I. Shulman. between pyruvate and oxaloacetate in awake normal and 3,3’-&triiodo-Lthyronine-treated rats. Am. J. Physiol. 267 (Endocrinol. Metab. 30): E273-E277, 1994.- between pyruvate and oxaloacetate was assessed in awake 24-h fasted normal and triiodothyronine (T&treated rats. After a 20- or 60-min infusion of 13-13C]alanine (99% enriched, 12 mg/min) the 13C enrichments of liver glucose and alanine carbons were analyzed by 1.3Cand lH nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. from phosphoenolpyruvate to pyruvate [via pyruvate kinase (PK)] and from oxaloacetate to pyruvate [via malic enzyme (ME)] relative to the pyruvate carboxylase (PC) flux [i.e., (PK + ME)/PC] was assessed by the ratio of the 13C enrichment of C-2 alanine relative to that in C-5 glucose. In the normal rats (PK + ME)/PC was 0.26 t 0.07 (n = 7, t = 20 min) and 0.37 * 0.08 (n = 4, t = 60 min). In the T3-treated rats the (PK + ME)/PC increased four- to fivefold to 1.03 t 0.19 (n = 8, t = 20 min) and to 1.83 2 0.19 (n = 3, t = 60 min) (P < 0.05 vs. normal rats). The

Journal

AJP - Endocrinology and Metabolism – The American Physiological Society

Published: Aug 1, 1994

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