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H1050 GeneraZ preparation. Experiments were performed on 17 mongrel dogs of either sex (body wt 13-18 kg) anesthetized with thiopental sodium (15 mg/kg iv) followed 15 min later by an 8% solution of cu-chloralose in polyglyco1 (50 mg/kg iv). Animals were ventilated mechanically on room air with positive pressure at a rate of 16-18 breaths/min and at a tidal volume of 20 ml/kg body wt. Arterial blood was withdrawn periodically for measurement of blood gases. Arterial 02 partial pressure was maintained above 80 mmHg, and arterial CO2 partial pressure was kept between 30 and 40 mmHg by adjusting the tidal volume. A 7.5% bicarbonate solution was administered intravenously, when needed, to maintain the pH between 7.30 and 7.40. Muscle movement during nerve dissection was prevented with gallamine triethiodide (Flaxedil, 3 mg/kg iv). Heparin (3 mg/kg iv) was given before cannulation of the blood vessels and then hourly (1 mg/kg iv). The dorsal branch of the left lateral saphenous vein was cannulated at the ankle and perfused at constant flow with a roller pump, using blood from the terminal aorta; a depulsator and heat exchanger were placed in the pump outflow line. To eliminate other sources of inflow to
AJP - Heart and Circulatory Physiology – The American Physiological Society
Published: Jun 1, 1982
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