“Woah! It's like Spotify but for academic articles.”

Instant Access to Thousands of Journals for just $30/month

Get 2 Weeks Free

Rat hepatic stellate cells produce cytokine-induced neutrophil chemoattractant in culture and in vivo

Rat hepatic stellate cells produce cytokine-induced neutrophil chemoattractant in culture and in vivo Abstract Hepatic stellate cells are widely recognized for their contribution to liver fibrosis. This study investigated whether these cells also promote hepatic inflammation by producing neutrophil chemoattractants. Specifically, stellate cells were examined as potential sources of cytokine-induced neutrophil chemoattractant (CINC), a rat chemokine resembling human interleukin-8. Stellate cells from normal rat liver expressed little or no CINC. In culture, CINC mRNA was induced rapidly, coinciding with the phenomenon of culture activation. CINC mRNA rose 4.6-fold within 3 days and was accompanied by secretion of immunoreactive and biologically active CINC protein (4.1 ng ⋅ μg DNA −1 ⋅ day −1 ). Studies in vivo demonstrated that CINC could be induced in stellate cells during liver injury. CINC mRNA rose significantly (4- to 6-fold) in two models of liver disease, both of which cause stellate cell activation. In summary, the data indicate that CINC is induced during stellate cell activation in culture and in vivo. They suggest that stellate cell-derived CINC can promote hepatic inflammation in vivo. chemokine chemotaxis leukocyte inflammation Footnotes Address for reprint requests: J. J. Maher, Liver Center Laboratory, San Francisco General Hospital, Bldg. 40, Rm. 4102, 1001 Potrero Ave., San Francisco, CA 94110. This work was supported by National Institutes of Health Grants AA-07810, AA-00215, and DK-26743 and the Alcoholic Beverage Medical Research Foundation. Portions of this work appeared earlier in abstract form ( Hepatology 24: 353A, 1996). Copyright © 1998 the American Physiological Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Gastrointestinal and Liver Physiology The American Physiological Society

Loading next page...
 
/lp/the-american-physiological-society/rat-hepatic-stellate-cells-produce-cytokine-induced-neutrophil-4Tf69Veftg

You're reading a free preview. Subscribe to read the entire article.

And millions more from thousands of peer-reviewed journals, for just $30/month

Get 2 Weeks Free

To be the best researcher, you need access to the best research

  • With DeepDyve, you can stop worrying about how much articles cost, or if it's too much hassle to order — it's all at your fingertips. Your research is important and deserves the top content.
  • Read from thousands of the leading scholarly journals from Springer, Elsevier, Nature, IEEE, Wiley-Blackwell and more.
  • All the latest content is available, no embargo periods.

Stop missing out on the latest updates in your field

  • We’ll send you automatic email updates on the keywords and journals you tell us are most important to you.
  • There is a lot of content out there, so we help you sift through it and stay organized.