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METHODS . OF ADRENOCORTICOTROPIN () from the anterior pituitary is stimulated by a number of hormonal factors (2, 4, 11, 44). There is considerable evidence that the 41-amino acid protein, corticotropin-releasing factor (CRF), is the most important of these factors and is necessary for a normal response to stress (4, 36). On the other hand, has been demonstrated to increase release both by directly stimulating pituitary secretion and also by potentiating the -releasing effect of CRF (2, 4, 11, 12, 36, 44). Although is considered a major stimulatory factor regulating secretion, indirect evidence SECRETION Experiments were performed on seven conscious male and female mongrel dogs weighing between 13.5 and 23.5 kg. The dogs were allowed free access to water and a standard dog chow (Ralston-Purina), which provided -75 meq sodium/day. Surgical preparation. Surgery was performed to implant catheters for arterial and atria1 pressure measurements and for intravenous infusions. Dogs were tranquilized with acepromazine maleate (0.5 mg/kg SC) and were anesthetized with thiamylal sodium (5 mg/kg iv). The dogs were then intubated, and anesthesia was maintained with halothane in nitrous oxide and 02. Polyvinyl chloride catheters (0.05 in. ID, 0,09 in. OD) were placed into the abdominal aorta and
AJP - Regulatory, Integrative and Comparative Physiology – The American Physiological Society
Published: Oct 1, 1988
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