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Intercellular Plasma of Centrifuged Human Erythrocytes as Measured by Means of Iodo 131 -Albumin

Intercellular Plasma of Centrifuged Human Erythrocytes as Measured by Means of Iodo 131 -Albumin METHOD Ten to twenty ml. samples of venous blood were drawn from 39 men and women, The blood was promptly mixed with heparin (I mg/Io ml. of blood). A 0.1 ml. volume of a saline solution of iodinated (1131) human serum albumin1 wasadded to each sample of heparinized blood and the flask* (50 ml.) was immediately stoppered. The 1131 activity was approximately 0.05 to OJ &ml. of whole blood. After a few minutes of gentle rotation of the blood an aliquot was removed (in duplicate) with a syringe pipet. The contents of the pipet and its washings were combined in a specially deReceived for publication July 30, 1952. l Obtained by authorization from the Atomic Energy Commission. 323 TABLE I. SUMMARY TRAPPED ..+ OF THE LITERATURE, IN PACKED CELLS INCLUDING PRESENT AFTER CENTRIFUGATION STUDIES, ON EXTENT OF WHOLE BLOOD PUSHA OF PLASMA -.-TRAPPING - .I % of AUTHOR SUE JECT METHOD DETERMINATIONS --- Pe~‘~$~e . -_. 2 c. _..6 ; heT;tUzri t . 2 ~- Keith et al. (I) Man , Man c. I.4 2b I --- c. c. -I IO IO Harris --Hooper Smith (3) el al. (4) (5) 81 TJampe (6) Dog -Dog Dw 1 Man http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Physiology The American Physiological Society

Intercellular Plasma of Centrifuged Human Erythrocytes as Measured by Means of Iodo 131 -Albumin

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Publisher
The American Physiological Society
Copyright
Copyright © 1953 the American Physiological Society
ISSN
8750-7587
eISSN
1522-1601
Publisher site
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Abstract

METHOD Ten to twenty ml. samples of venous blood were drawn from 39 men and women, The blood was promptly mixed with heparin (I mg/Io ml. of blood). A 0.1 ml. volume of a saline solution of iodinated (1131) human serum albumin1 wasadded to each sample of heparinized blood and the flask* (50 ml.) was immediately stoppered. The 1131 activity was approximately 0.05 to OJ &ml. of whole blood. After a few minutes of gentle rotation of the blood an aliquot was removed (in duplicate) with a syringe pipet. The contents of the pipet and its washings were combined in a specially deReceived for publication July 30, 1952. l Obtained by authorization from the Atomic Energy Commission. 323 TABLE I. SUMMARY TRAPPED ..+ OF THE LITERATURE, IN PACKED CELLS INCLUDING PRESENT AFTER CENTRIFUGATION STUDIES, ON EXTENT OF WHOLE BLOOD PUSHA OF PLASMA -.-TRAPPING - .I % of AUTHOR SUE JECT METHOD DETERMINATIONS --- Pe~‘~$~e . -_. 2 c. _..6 ; heT;tUzri t . 2 ~- Keith et al. (I) Man , Man c. I.4 2b I --- c. c. -I IO IO Harris --Hooper Smith (3) el al. (4) (5) 81 TJampe (6) Dog -Dog Dw 1 Man

Journal

Journal of Applied Physiology – The American Physiological Society

Published: Jan 1, 1953

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