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In vivo detection of endogenous acetylcholine release in cat ventricles

In vivo detection of endogenous acetylcholine release in cat ventricles Akiyama, Tsuyoshi, Toji Yamazaki, and Ishio Ninomiya. In vivo detection of in cat ventricles. Am. J. Physiol. 266 ( Circ. PhysioZ. 35): H854-H860, 1994.-To detect and monitor (ACh) in vivo , we applied a dialysis technique to the s of anesthetized cats. Dialysis probes were implanted left ventricular myocardium and were perfused with Krebs-Henseleit solution containing Eserine (lop4 M) at 3 pl/min. Dialysate ACh concentration was measured with high-performance liquid chromatography. In four cats, the response to vagal stimulation was studied. Electrical stimulation of efferent vagal nerves (10 Hz) significantly increased dialysate ACh concentration from 596 t 118 (control) to 12,210 t 1,661 pM. After stimulation, dialysate ACh concentration significantly decreased to 382 or 80 pM below control. The influence of ganglionic blocker was determined in six cats. Control vagal nerve stimulation (10 Hz) increased dialysate ACh concentration from 582 * 136 to 9,102 * 754 PM. Local perfusion of hexamethonium ( 10Y4 M) did not affect this nerve stimulationinduced ACh increase (8,611 t 1,189 PM), and intravenous administration of hexamethonium (20 mg/kg) prevented this increase (340 t 88 PM). We examined the response to vagal nerve stimulation at different frequencies in three cats. Vagal nerve stimulation increased http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Heart and Circulatory Physiology The American Physiological Society

In vivo detection of endogenous acetylcholine release in cat ventricles

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Publisher
The American Physiological Society
Copyright
Copyright © 1994 the American Physiological Society
ISSN
0363-6135
eISSN
1522-1539
Publisher site
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Abstract

Akiyama, Tsuyoshi, Toji Yamazaki, and Ishio Ninomiya. In vivo detection of in cat ventricles. Am. J. Physiol. 266 ( Circ. PhysioZ. 35): H854-H860, 1994.-To detect and monitor (ACh) in vivo , we applied a dialysis technique to the s of anesthetized cats. Dialysis probes were implanted left ventricular myocardium and were perfused with Krebs-Henseleit solution containing Eserine (lop4 M) at 3 pl/min. Dialysate ACh concentration was measured with high-performance liquid chromatography. In four cats, the response to vagal stimulation was studied. Electrical stimulation of efferent vagal nerves (10 Hz) significantly increased dialysate ACh concentration from 596 t 118 (control) to 12,210 t 1,661 pM. After stimulation, dialysate ACh concentration significantly decreased to 382 or 80 pM below control. The influence of ganglionic blocker was determined in six cats. Control vagal nerve stimulation (10 Hz) increased dialysate ACh concentration from 582 * 136 to 9,102 * 754 PM. Local perfusion of hexamethonium ( 10Y4 M) did not affect this nerve stimulationinduced ACh increase (8,611 t 1,189 PM), and intravenous administration of hexamethonium (20 mg/kg) prevented this increase (340 t 88 PM). We examined the response to vagal nerve stimulation at different frequencies in three cats. Vagal nerve stimulation increased

Journal

AJP - Heart and Circulatory Physiology – The American Physiological Society

Published: Mar 1, 1994

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