Abstract Human-induced pluripotent stem cells (hiPSCs) can differentiate into functional cardiomyocytes; however, the electrophysiological properties of hiPSC-derived cardiomyocytes have yet to be fully characterized. We performed detailed electrophysiological characterization of highly pure hiPSC-derived cardiomyocytes. Action potentials (APs) were recorded from spontaneously beating cardiomyocytes using a perforated patch method and had atrial-, nodal-, and ventricular-like properties. Ventricular-like APs were more common and had maximum diastolic potentials close to those of human cardiac myocytes, AP durations were within the range of the normal human electrocardiographic QT interval, and APs showed expected sensitivity to multiple drugs (tetrodotoxin, nifedipine, and E4031). Early afterdepolarizations (EADs) were induced with E4031 and were bradycardia dependent, and EAD peak voltage varied inversely with the EAD take-off potential. Gating properties of seven ionic currents were studied including sodium ( I Na ), L-type calcium ( I Ca ), hyperpolarization-activated pacemaker ( I f ), transient outward potassium ( I to ), inward rectifier potassium ( I K1 ), and the rapidly and slowly activating components of delayed rectifier potassium ( I Kr and I Ks , respectively) current. The high purity and large cell numbers also enabled automated patch-clamp analysis. We conclude that these hiPSC-derived cardiomyocytes have ionic currents and channel gating properties underlying their APs and EADs that are quantitatively similar to those reported for human cardiac myocytes. These hiPSC-derived cardiomyocytes have the added advantage that they can be used in high-throughput assays, and they have the potential to impact multiple areas of cardiovascular research and therapeutic applications. patch clamp ion channels iCell cardiomyocytes Copyright © 2011 the American Physiological Society « Previous | Next Article » Table of Contents This Article Published online before print September 2011 , doi: 10.​1152/​ajpheart.​00694.​2011 AJP - Heart November 2011 vol. 301 no. 5 H2006-H2017 » Abstract Free Full Text Free to you Full Text (PDF) Free to you Supplemental Tables All Versions of this Article: ajpheart.00694.2011v1 301/5/H2006 most recent Classifications Cardiac Excitation and Contraction Services Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Citing Articles Load citing article information Citing articles via Web of Science Google Scholar Articles by Ma, J. Articles by January, C. T. PubMed PubMed citation Articles by Ma, J. Articles by January, C. T. Related Content Cardiac Excitation and Contraction Load related web page information Current Issue November 2011, 301 (5) Alert me to new issues of AJP - Heart About the Journal Information for Authors Submit a Manuscript Ethical Policies AuthorChoice PubMed Central Policy Reprints and Permissions Advertising Press Copyright © 2011 the American Physiological Society Print ISSN: 0363-6135 Online ISSN: 1522-1539 var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-2924550-1"); pageTracker._trackPageview();

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